Efeito do extrato hidroalcoólico de folhas de Leucaena leucocephala sobre o nascimento do Haemonchus contortus in vitro
Palavras-chave:
Leucaena leucocephala, extrato hidroalcoólico, atividade ovicida, Haemonchus contortusResumo
Haemonchus contortus (HC) é um nemátodo gastrintestinal de importância mundial devido às perdas econômicas que causa direta e indiretamente na produção ovina. Extratos vegetais ricos em metabólitos secundários têm sido propostos como um método alternativo de controle de nematóides gastrintestinais em ovinos e caprinos, pois cumprem sua função biológica e proporcionam um acompanhamento ambiental sustentável. O objetivo do presente experimento foi determinar o efeito do extrato hidroalcoólico de folhas de Leucaena leucocephala (EHLL) na eclosão do Haemonchus contortus in vitro. O extrato foi obtido pela técnica de maceração e foi submetido à caracterização fitoquímica qualitativa, na qual foram identificadas instaurações, oxidrils fenólicos, lactonas, flavonóis, florataninas, cumarinas, saponinas e compostos aromáticos. Concentrações de 100, 90, 80, 80, 80, 70, 70, 60 e 50 mg/mL do extrato foram avaliadas sobre a inibição de eclosão de ovos (IEH) de Haemonchus contortus. O extrato hidroalcoólico de L. leucocephala mostrou 71% IEH a 100 mg/mL, um CE50 e CE90 de 52,22 e 302 mg/mL respectivamente. O extrato hidroalcoólico de folhas de Leucaena leucocephala contém compostos bioativos (fenólicos, terpenos e flavonóides) que inibem o nascimento de ovos de HC com efeito dose-dependente. Portanto, a EHLL poderia ser considerada como uma alternativa para controlar este nematódeo, entretanto, é necessário determinar o metabolito específico que confere a atividade, determinar sua toxicidade e realizar ensaios in vivo.
e2021-54
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