Efecto antihelmíntico y antibacteriano in vitro del extracto hidroalcohólico de hojas y tallos de Kalanchoe daigremontiana

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Palabras clave:

Kalanchoe daigremontiana, extracto hidroalcohólico, antihelmíntico, antibacteriano

Resumen

Cinco concentraciones (200 a 12.5 mg mL -1 ) del extracto hidroalcohólico de Kalanchoe daigremontiana  fueron usadas para determinar su efecto antihelmíntico y antibacteriano in vitro. Para determinar el efecto antihelmíntico se realizaron las pruebas de inhibición de la eclosión, inhibición de la motilidad y mortalidad larvaria sobre Haemonchus contortus (HC). La determinación de la Concentración Mínima Inhibitoria y Concentración Mínima Bactericida, permitieron evaluar la actividad antibacteriana del extracto sobre L. monocytogenes, S. typhi, P. aeruginosa, S. choleraesuis, B. subtilis, E. coli and S. aureus. El extracto hidroalcohólico de Kalanchoe daigremontiana a 400 mg mL -1 inhibió la eclosión de huevos de HC en un 99.5%, y redujo la motilidad del 85.2% de larvas L3 del mismo nematodo. Las concentraciones letales 50 y 90 para la inhibición de la eclosión fueron de 66.5 y 87.3 mg mL -1 y de 1.5 y 240.9 mg mL -1, para la inhibición de la motilidad. El extracto mostró actividad sobre bacterias Gram positivas y Gram negativas, determinándose una MIC de 100 mg mL −1 sobre P. aeruginosa  L. monocytogenes y 0.781 mg mL −1 para B. subtilis and S. aureus. Estos resultados indican que el extracto hidroalcohólico de Kalanchoe daigremontiana presenta potencial efecto antihelmíntico y antibacteriano y que podría ser utilizado como una estrategia de control biológico.

http://dx.doi.org/10.21929/abavet2022.1   

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Citas

ABDELFATTA S, Elghandour M, Kholif A, López S, Pliego A, Cipriano-Salazar M, Alonso M. 2017. Tree leaves of Salix babylonica extract as a natural anthelmintic for small-ruminant farms in a semiarid region in Mexico. Agroforestry Systems. 91(1):111-122. ISSN: 1572-9680. https://doi.org/10.1007/s10457-016-9909-z

AGUILAR-Marcelino L, Mendoza-De-Give P, Torres-Hernández G, López-Arellano M, Becerril-Pérez C, Orihuela-Trujillo A, Olmedo-Juárez A. 2016. Consumption of nutritional pellets with Duddingtonia flagrans fungal chlamydospores reduces infective nematode larvae of Haemonchus contortus in faeces of Saint Croix lambs. Journal Helminthology. 91(6): 665-671. ISSN: 1475-2697. https://doi.org/10.1017/S0022149X1600081X

AKINNIBOSUN FI, Eedionwe O. 1994. Evaluation of the phytochemical and antimicrobial potential of the leaves extracts of Bryophyllum pinnatum L. and Citrus aurantifolia Sw and their synergy. Journal of Applied Sciences and Environmental Management. 4:611-619. ISSN: 2659-1502. http://dx.doi.org/10.4314/jasem.v19i4.7

ALCALA-Canto Y, Camberos L, López H, Olvera L, Pérez G. 2016 Anthelmintic resistance status of gastrointestinal nematodes of sheep to the single or combined administration of benzimidazoles and closantel in three localities in Mexico. Veterinaria México. 3(4). ISSN: 2448-6760. https://doi.org/10.21753/vmoa.3.4.374

AISYAH LS, Yun YF, Herlina T, Julaeha E, Zainuddin A, Nurfarida I, Shiono Y. 2017. Flavonoid compounds from the leaves of Kalanchoe prolifera and their cytotoxic activity against P-388 murine leukimia cells. Natural Product Sciences. 23(2):139-145. ISSN: 2288-9027. https://doi.org/10.20307/nps.2017.23.2.139

AKKARI H, Hajaji S, B'Chir F, Rekik M, Gharbi M. 2016. Correlation of polyphenolic content with radical-scavenging capacity and anthelmintic effects of Rubus ulmifolius (Rosaceae) against Haemonchus contortus. Veterinary Parasitology. 221:46-53. ISSN: 0304-4017. https://doi.org/10.1016/j.vetpar.2016.03.007

BASHIR L, Chishti M, Bhat F, Tak H, Bandh S, Khan A. 2017. Evaluation of anthelmintic antimicrobial and antioxidant activity of Chenopodium album. Tropical Animal Health and Production. 49(8):1597-1605. ISSN: 1573-7438. https://doi.org/10.1007/s11250-017-1364-y

CASTILLO-MITRE G, Olmedo-Juárez A, Rojo-Rubio R, González-Cortázar M, Mendoza-de Givesh P, Hernández-Beteta E, Zamilpa A. 2017. Caffeoyl and coumaroyl derivatives from Acacia cochliacantha exhibit ovicidal activity against Haemonchus contortus. Journal of Ethnopharmacology. 204: 125-131. ISSN: 0378-8741.

https://doi.org/10.1016/j.jep.2017.04.010

CORDERO CM, Rojo A, Hernández R, Calvalho V. Parasitología Veterinaria. Barcelona, España. Editorial McGraw-Hill Interamericana. 2000.ISBN: 84-486-0236-6 https://dialnet.unirioja.es/servlet/libro?codigo=489596

CRYR M, Lane K, Greer M, Cates R, Burt S, Andrus M, Johnson FB. 2017. Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity. Pharmaceutical biology. 55(1):1586-1591. PMID: 28395583. https://doi.org/10.1080/13880209.2017.1310907

DE SOUZA BARBOZA TJE, Ferreira AEF, Ignacio ACDPR, Albarello N. 2016. Cytotoxic, antibacterial and antibiofilm activities of aqueous extracts of leaves and flavonoids occurring in Kalanchoe pinnata (Lam.) Pers. Journal of Medicinal Plants Research. 10(41):763-770. https://doi.org/10.5897/JMPR2016.6260

GEORGE LO, Radha HR, Somasekariah BV. 2018. In vitro anti-diabetic activity and GC-MS analysis of bioactive compounds present in the methanol extract of Kalanchoe pinnata. Indian Journal of Chemistry. 57: 1213-1221.

https://api.semanticscholar.org/CorpusID:91497628

HERNÁNDEZ-ALVARADO J, Zaragoza-Bastida A, López-Rodríguez G, Peláez-Acero A, Olmedo-Juárez A, Rivero-Pérez N. 2018. Antibacterial and antihelmintic activity of plant secondary metabolites: approach in Veterinary Medicine. Abanico Veterinario. 8:14-27. ISSN: 2448-6132. https://doi.org/10.21929/abavet2018.81.1

HERNÁNDEZ-Villegas M, Borges-Argaez R, Rodríguez-Vivas R, Torres-Acosta J, Méndez-González M, Cáceres-Farfán M. 2011. Ovicidal and larvicidal activity of the crude extracts from Phytolacca icosandra against Haemonchus contortus. Veterinary Parasitology. 179 (1-3):100-106. ISSN: 0304-4017.

https://doi.org/10.1016/j.vetpar.2011.02.019

HUANG HC, Lin MK, Yang HL, Hseu YC, Liaw CC, Tseng YH, Kuo YH. 2013. Cardenolides and bufadienolide glycosides from Kalanchoe tubiflora and evaluation of cytotoxicity. Planta Medica. 79(14):1362-1369. ISSN: 0032-0943.

https://doi.org/10.1055/s-0033-1350646

IRSHAD M, Man S, Rizvi MA. 2010. Assessment of anthelmintic activity of Cassia fistula L. Middle East Journal of Scientific Research. 5(5):346-349. IISSN:1999-8147. http://www.idosi.org/mejsr/mejsr5(5)/5.pdf

JIAO YQ, Preston S, Song HJ, Jabbar A, Liu YX, Baell J, Gasser RB. 2017. Assessing the anthelmintic activity of pyrazole-5-carboxamide derivatives against Haemonchus contortus. Parasit Vectors. 10:214-234. ISSN: 1756-3305.

https://doi.org/10.1186/s13071-017-2191-8

KARWATZKI B, Herget A, Beerhues L, Wiermann R. 1993. In-situ localization of chalcone synthase in tannin-containing plants. Phytochemistry. 32(3):585. ISSN: 0031-9422. https://doi.org/10.1016/S0031-9422(00)95141-0

KOLODZIEJCZYK-CZEPAS J, Stochmal A. 2017. Bufadienolides of Kalanchoe species: an overview of chemical structure, biological activity and prospects for pharmacological use. Phytochemistry Reviews. 16(6):1155-1171. ISSN: 1572-980X.

https://dx.doi.org/10.1007%2Fs11101-017-9525-1

KOLODZIEJCZYK-CZEPAS J, Nowak P, Wachowicz B, Piechocka J, Głowacki R, Moniuszko-Szajwaj B, Stochmal A. 2016. Antioxidant efficacy of Kalanchoe daigremontiana bufadienolide-rich fraction in blood plasma in vitro. Pharmaceutical biology. 54(12): 3182-3188. PMID: 27488985.

https://doi.org/10.1080/13880209.2016.1214740

LÓPEZ-AROCHE U, Salinas-Sánchez D, de Gives P, López-Arellano M, Liebano-Hernández E, Valladares-Cisneros G, Hernandez-Velázquez V. 2008. In vitro nematicidal effects of medicinal plants from the Sierra de Huautla, Biosphere Reserve, Morelos, México against Haemonchus contortus infective larvae. Journal of Helminthology. 82(1):25-31. ISSN:1475-2697 https://doi.org/10.1017/S0022149X07873627

LUNKAD AS, Agrawal MR, Kothawade SN. 2016. Anthelmintic activity of Bryophyllum pinnatum. Research Journal of Pharmacognosy and Phytochemistry. 8(1):21-24. https://doi.org/10.5958/0975-4385.2016.00005.4

MOTHANA R, Lindequist U, Gruenert R, Bednarski JP. 2009. Studies of the in vitro anticancer, antimicrobial and antioxidant potentials of selected Yemeni medicinal plants from the island Soqotra. BMC Complementary Medicine and Therapies. 9:7. ISSN: 2662-7671. https://doi.org/10.1186/1472-6882-9-7

MORADPOUR N, Borji H, Razmi G, Maleki M, Kazemi H. 2013. Pathophysiology of Marshallagia marshalli in experimentally infected lambs. Parasitology. ISSN: 1469-8161 140(14):1762-1767. https://doi.org/10.1017/s0031182013001042

MUZITANO MF, Tinoco LW, Guette C, Kaiser CR, Rossi-Bergmann B, Costa SS. 2006. The antileishmanial activity assessment of unusual flavonoids from Kalanchoe pinnata. Phytochemistry. 67(18): 2071-2077. PMID: 16930642.

https://doi.org/10.1016/j.phytochem.2006.06.027

OLMEDO-Juárez A, Rojo-Rubio R, Zamilpa A, de Gives PM, Arece-García J, López-Arellano ME, von Son-de Fernex E. 2017. In vitro larvicidal effect of a hydroalcoholic extract from Acacia cochliacantha leaf against ruminant parasitic nematodes. Veterinary Research Communications. 41 (3):227-232. ISSN :1573-7446.

https://doi.org/10.1007/s11259-017-9687-8

PHATAK RS. 2016. Lack of anthelmintic activity of Kalanchoe pinnata fresh leaves. Journal of Pharmaceutical Negative Results. 7(1):21. ISSN: 2229-7723. https://www.pnrjournal.com/fulltext/219-1599738067.pdf?1623436039

PRASAD PB, Prakash S, Adhikari K. 2020. LC‐ESI‐QTOF‐MS for the Profiling of the Metabolites and in Vitro Enzymes Inhibition Activity of Bryophyllum pinnatum and Oxalis corniculata Collected from Ramechhap District of Nepal. Chemistry & biodiversity. 17(6): e2000155. https://doi.org/10.1002/cbdv.202000155

PREET S, Tomar R. 2017. Anthelmintic effect of biofabricated silver nanoparticles using Ziziphus jujuba leaf extract on nutritional status of Haemonchus contortus. Small Ruminant Research. 154:45-51. ISSN: 0921-4488.

https://doi.org/10.1016/j.smallrumres.2017.07.002

RICHWAGEN N, LYLES JT, Dale BL, Quave CL. 2019. Antibacterial activity of Kalanchoe mortagei and K. fedtschenkoi against ESKAPE pathogens. Frontiers in pharmacology, 10, 67. https://doi.org/10.3389/fphar.2019.00067

RIVERO-PÉREZ N, Ayala-Martinez M, Zepeda-Bastida A, Meneses-Mayo M, Ojeda-Ramirez D. 2016. Anti-inflammatory effect of aqueous extracts of spent Pleurotus ostreatus substrates in mouse ears treated with 12-O-tetradecanoylphorbol-13-acetate. The Indian Journal of Pharmacology. 48:141-144. ISSN: 0253-7613.

https://ijp-online.com/article.asp?issn=0253-7613;year=2016;volume=48;issue=2;spage=141;epage=144;aulast=Rivero-Perez;type=0

SANTOS FO, de Lima HG, Santos N, Serra T, Uzeda RS, Reis IMA. 2017. Batatinha, MJM. In vitro anthelmintic and cytotoxicity activities the Digitaria insularis (Poaceae). Veterinary Parasitology. 245:48-54. ISSN:0304-4017.

https://doi.org/10.1016/j.vetpar.2017.08.007.

SANOS S, Zurfluh L, Mennet M, Potterat O, Von Mandach U, Hamburger M, Simões-Wüst, AP. 2021. Bryophyllum pinnatum Compounds Inhibit Oxytocin-Induced Signaling Pathways in Human Myometrial Cells. Frontiers in Pharmacology.12:142.

https://doi.org/10.3389/fphar.2021.632986

SINGAB AB, El-Ahamdy SH, Labib RM, Fekry SS. Kalanchoe thrysiflora Harv. And Kalanchoe marmorata Baker; DNA Profiling, biological guided fractionation of different extracts; isolation and identification of cytotoxic compounds. 2012. Journal of Applied Pharmaceutical Science. 02 (08): 215-220. ISSN: 2231-3354.

http://japsonline.com/admin/php/uploads/652_pdf.pdf

SUPRATMAN U, Fujita T, Akiyama K, Hayashi H. 2000. New insecticidal bufadienolide, bryophyllin C, from Kalanchoe pinnata. Bioscience, biotechnology, and biochemistry. 64(6): 1310-1312. PMID: 10923811. https://doi.org/10.1271/bbb.64.1310

SUPRATMAN, U, Fujita T, Akiyama K, Hayashi H. 2001. Insecticidal compounds from Kalanchoe daigremontiana× tubiflora. Phytochemistry. 58(2): 311-314. PMID: 11551556 https://doi.org/10.1016/S0031-9422(01)00199-6

SUPRATMAN U, Fujita T, Akiyama K, Hayashi H, Murakami A, Sakai H, Ohigashi H. 2001. Anti-tumor Promoting Activity of Bufadienolides from Kalanchoe pinnata and K. daigremontiana× butiflora. Bioscience, biotechnology, and biochemistry. 65(4): 947-949. PMID: 11388478. https://doi.org/10.1271/bbb.65.947

STEFANOWICZ-HAJDUK J, Asztemborska M, Krauze-Baranowska M, Godlewska S, Gucwa M, Moniuszko-Szajwaj B, Ochocka JR. 2020. Identification of flavonoids and bufadienolides and cytotoxic effects of Kalanchoe daigremontiana extracts on human cancer cell lines. Planta medica. 86(04): 239-246. PMID: 31994149.

https://doi.org/10.1055/a-1099-9786

STEFANOWICZ-HAJDUK J, Hering A, Gucwa M, Hałasa R, Soluch A, Kowalczyk M, Ochocka R. 2020. Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content. Pharmaceutical Biology. 58(1):732-740. PMID: 32715869. https://doi.org/10.1080/13880209.2020.1795208

XING LZ, Ni HJ, Wang L. 2017. Quercitrin attenuates osteoporosis in ovariectomized rats by regulating mitogen-activated protein kinase (MAPK) signaling pathways. Biomedicine & Pharmacotherapy: 89: 1136-1141. PMID: 28314242.

https://doi.org/10.1016/j.biopha.2017.02.073

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2022-03-04

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