Efecto antihelmíntico y antibacteriano in vitro del extracto hidroalcohólico de hojas y tallos de Kalanchoe daigremontiana
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Kalanchoe daigremontiana, extracto hidroalcohólico, antihelmíntico, antibacterianoResumen
Cinco concentraciones (200 a 12.5 mg mL -1 ) del extracto hidroalcohólico de Kalanchoe daigremontiana fueron usadas para determinar su efecto antihelmíntico y antibacteriano in vitro. Para determinar el efecto antihelmíntico se realizaron las pruebas de inhibición de la eclosión, inhibición de la motilidad y mortalidad larvaria sobre Haemonchus contortus (HC). La determinación de la Concentración Mínima Inhibitoria y Concentración Mínima Bactericida, permitieron evaluar la actividad antibacteriana del extracto sobre L. monocytogenes, S. typhi, P. aeruginosa, S. choleraesuis, B. subtilis, E. coli and S. aureus. El extracto hidroalcohólico de Kalanchoe daigremontiana a 400 mg mL -1 inhibió la eclosión de huevos de HC en un 99.5%, y redujo la motilidad del 85.2% de larvas L3 del mismo nematodo. Las concentraciones letales 50 y 90 para la inhibición de la eclosión fueron de 66.5 y 87.3 mg mL -1 y de 1.5 y 240.9 mg mL -1, para la inhibición de la motilidad. El extracto mostró actividad sobre bacterias Gram positivas y Gram negativas, determinándose una MIC de 100 mg mL −1 sobre P. aeruginosa y L. monocytogenes y 0.781 mg mL −1 para B. subtilis and S. aureus. Estos resultados indican que el extracto hidroalcohólico de Kalanchoe daigremontiana presenta potencial efecto antihelmíntico y antibacteriano y que podría ser utilizado como una estrategia de control biológico.
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