Efeito anti-helmíntico e antibacteriano in vitro do extracto hidroalcoólico de folhas e caules de Kalanchoe daigremontiana
Palavras-chave:
Kalanchoe daigremontiana, extracto hidroalcoólico, anti-helmíntico, antibacterianoResumo
Cinco concentrações (200 a 12,5 mg mL-1
) do extracto hidroalcoólico de Kalanchoe daigremontiana foram
utilizadas para determinar o seu efeito anti-helmíntico e antibacteriano in vitro. Para determinar o efeito
anti-helmíntico, foram realizados testes de inibição de eclosão, inibição de motilidade e mortalidade larvar
em Haemonchus contortus (HC). A determinação da Concentração Inibitória Mínima e da Concentração
Bactericida Mínima permitiu a avaliação da actividade antibacteriana do extracto em L. monocytogenes, S.
typhi, P. aeruginosa, S. choleraesuis, B. subtilis, E. coli e S. aureus. O extracto hidroalcoólico de Kalanchoe
daigremontiana a 400 mg mL -1 inibiu a eclosão dos ovos de HC em 99,5%, e reduziu a motilidade de
85,2% das larvas de L3 do mesmo nemátodo. As concentrações de 50 e 90 concentrações letais para
inibição de eclosão foram 66,5 e 87,3 mg mL-1 e 1,5 e 240,9 mg mL-1
, para inibição da motilidade. O extracto
mostrou actividade nas bactérias Gram-positivas e Gram-negativas, determinando um CIM de 100 mg mL1 em P. aeruginosa e L. monocytogenes e 0,781 mg mL -1 para B. subtilis e S. aureus. Estes resultados
indicam que o extracto hidroalcoólico de Kalanchoe daigremontiana tem um potencial efeito anti-helmíntico
e antibacteriano e poderia ser utilizado como estratégia de controle biológico.
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