Efeito decapacitante das proteínas oviductais em espermatozóides de galo in vitro
Palavras-chave:
adenosina trifosfato, glutationa reduzida, malondialdeído, oxidação, espermatozóidesResumo
Em aves domésticas, o processo de maturação dos espermatozoides não foi totalmente descrito. O objetivo do estudo foi determinar os parâmetros in vitro do efeito incapacitante das proteínas oviductais sobre os espermatozóides de galos. Alíquotas com espermatozoides foram incubadas in vitro para induzir estados metabólicos de capacitação, desabilitação e reação acrossomal para determinar as porcentagens de espermatozoides vivos, sua motilidade e concentrações de malondialdeído, glutationa reduzida e trifosfato de adenosina como parâmetros do estado metabólico dos espermatozoides. Os resultados mostraram porcentagens de motilidade e espermatozóides vivos no sêmen fresco e capacitado semelhantes (P>0,05), mas maiores (P<0,05) do que as determinadas em espermatozóides com reação acrossomal. O nmol/ml de MDA no sêmen fresco (1,59) foi maior (P<0,005) do que nos espermatozóides capacitados (1,05), com reação acrossomal (1,07) e não capacitados (1,05). Os nmol/ml de GSG foram semelhantes (P>0,05) em sêmen fresco (72,6) e capacitado (62,04), e entre sêmen com reação acrossomal (99,09) e não capacitado (86,07). A maior concentração de ATP foi no sêmen capacitado com 69,9 μmol/ml, com concentrações semelhantes (P<0,05) no sêmen fresco, com reação acrossomal e não capacitado. Os parâmetros determinados mostraram que a fração proteica da junção útero-vaginal, in vitro, produz decapacitação e mantém a viabilidade dos espermatozoides.
http://dx.doi.org/10.21929/abavet2023.13
e2022-77
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