Decapacitating effect of oviductal proteins in rooster spermatozoa in vitro
Keywords:
Adenosine triphosphate, reduced glutathione, malondialdehyde, oxidation, semenAbstract
In birds, the process of sperm maturation has not been fully described. The objective of this study was to determine in vitro parameters for the decapacitating effect of oviductal proteins on rooster spermatozoa. Aliquots with spermatozoon were incubated to in vitro induce metabolic states: capacitation, decapacitation, and the acrosomal reaction to again determined percentages of live spermatozoa, motility, and concentrations of Malondialdehyde, reduced Glutathione and Adenosine triphosphate. The percentages of motility and live sperm in fresh and capacitated semen were similar (P>0.05), but higher (P<0.05) than those determined in decapacitated semen and with acrosome reaction. The determination of nmol/ml of MDA in fresh semen (1.59) was higher (P<0.005) than in capacitated semen (1.05), with acrosomal reaction (1.07) and decapacitated semen (1.05). GSH nmol/ml were similar (P>0.05) in fresh (72.6) and capacitated (62.04) semen, similarly (P<0.05) between acrosome reaction (99.09) and decapacitated (86.07) semen. The highest concentration of ATP was in capacitated semen with 69.9 µmol/ml, with similar concentrations (P<0.05) in fresh, with acrosomal reaction, and decapacitated semen. The parameters, determined, demonstrated that the protein fraction of the utero-vaginal junction, in vitro, produces decapacitation and successfully maintains sperm viability.
http://dx.doi.org/10.21929/abavet2023.13
e2022-77
https://www.youtube.com/watch?v=0rYTQI3wmoU
References
ÁLVAREZ-RODRÍGUEZ M, Ntzouni M, Wright D, Khan KI, López-Béjar M, Martínez CA, Rodríguez-Martínez H. 2020. Chicken seminal fluid lacks CD9- and CD44-bearing extracellular vesicles. Reprod Domest Anim. 55(3):293-300. ISSN: 439-0531. https://doi.org/10.1111/rda.13617
ASANO A, Tajima A. 2017. Development and Preservation of Avian Sperm. Adv Exp Med Biol. 1001:59-73. ISSN: 2214-8019. https://doi.org/10.1007/978-981-10-3975-1_4
APPLEGATE TJ, Angel R. 2014. Nutrient requirements of poultry publication: History and need for an update. J Appl Poult Res. 23(3): 567-575. ISSN: 1056-6171. https://doi.org/10.3382/japr.2014-00980
ASHRAF S, Bhatti SA, Nawaz H, Khan MS. 2020. Assessment of dietary selenium sources in commercial male broiler breeders: effects on semen quality, antioxidant status and immune responses. Pak Vet J. 40(1): 13-18. ISSN 2074-7764.
http://www.pvj.com.pk/abstract/40_1/19-130.htm
BAKST M. 2010. Role of the oviduct in maintaining sustained fertility in hens. J Anim. 89(5): 1323-1329. ISNN: 1525-3163. https://doi.org/10.2527/jas.2010-3663
CAMARILLO R, Jiménez I, Guzmán A, Rosales A, Rodríguez F, Pérez-Rivero JJ, Herrera JA. 2019. Oviductal proteins effect in rooster spermatic cryopreservation. CryoLetters. 40(6): 352-356. ISSN: 0143-2044.
http://www.cryoletters.org/Abstracts/vol_40_6_2019.htm#352
DÁVILA PM, Martin M P, Tapia JA, Ortega F C, Balao C C, Peña FJ. 2015. Inhibition of Mitochondrial Complex I Leads to Decreased Motility and Membrane Integrity Related to Increased Hydrogen Peroxide and Reduced ATP Production, while the Inhibition of Glycolysis Has Less Impact on Sperm Motility. PLoS One. 10(9):e0138777. ISSN: 1932-6203. https://doi.org/10.1371/journal.pone.0138777
FATTAH A, Sharafi M, Masoudi R, Shaverdi A, Esmaeili V. 2017. L-carnitina is a survival factor for chilled storage of rooster semen for a long time. Cryobiology. 74:13-18. ISSN: 1090-2392. https://doi.org/10.1016/j.cryobiol.2016.12.011
FERRAMOSCA A, Zara V. 2014. Bioenergetics of mammalian sperm capacitation. Biomed Res Int. 2014:902953. ISSN: 2314-6141. https://doi.org/10.1155/2014/902953
FISCHER DH, Failing SK, Meinecke‐Tillmann S, Wehrend A, Lierz M. 2020. Viability assessment of spermatozoa in large falcons (Falco spp.) using various staining protocols. Reprod Domestic Anim. 55(10):1383-1392.ISSN: 1439-0531.
https://doi.org/10.1111/rda.13785
GONZÁLEZ-SANTOS, Jorge A, Ávalos-Rodríguez, Alejandro, Martínez-García, José A, Rosales-Torres, Ana M, Herrera-Barragán, José A. 2019. Sperm morphophysiology in different sections of the rooster reproductive tract. Int J Morphol, 37(3): 861-866. ISSN: 0717-9502. https://dx.doi.org/10.4067/S0717-95022019000300861
HAMMER Ø, Harper DAT and Ryan PD. 2001. Past: Paleontological statistics software package for education and data analysis. Palaeontol Electron. 4(1):1–9. ISSN: 1094-8074. https://palaeo-electronica.org/2001_1/past/past.pdf
ITO T, Yoshizaki N, Tokumoto T, Ono H, Yoshimura T, Tsukada A, Kansaku N, Sasanami T. 2011. Progesterone is a sperm-releasing factor from the sperm-storage tubules in birds. Endocrinology. 152(10):3952–3962. ISSN: 1945-7170. https://doi.org/10.1210/en.2011-0237
JABBAR A, Abbass W, Riaz A, Sattar A, Akram M. 2015. Effect of different concentrations of ascorbic acid on semen quality and hatchability of indigenous aseel chicken. J Anim Plant Sci. 25(5):1222-1226. ISSN: 1018-7081. http://www.thejaps.org.pk/docs/v-25-05/03.pdf
KU HK, Lim HM, Oh KH, Yang HJ, Jeong JS, Kim SK. 2013. Interpretation of protein quantitation using the Bradford assay: comparison with two calculation models. Anal Biochem. 434(1):178-80. ISSN: 1096-0309. https://doi.org/10.1016/j.ab.2012.10.045
KURKOWSKA W, Bogacz A, Janiszewska M, Gabryś E, Tiszler M, Bellanti F, Kasperczyk S, Machoń-Grecka A, Dobrakowski M, Kasperczyk A. 2020. Oxidative stress is associated with reduced sperm motility in normal semen. Am J Mens Health. 14(5):1-8. ISSN: 1557-9891. https://doi.org/10.1177/1557988320939731
LEMOINE MS, Mignon-Grasteau I, Grasseau M, Magistrini E, Bleisbois E. 2011. Ability of chicken spermatozoa to undergo acrosome reaction after liquid storage or cryopreservation. Theriogenology. 75(1):122-130. ISSN: 1879-3231.
https://doi.org/10.1016/j.theriogenology.2010.07.017
LONG J, Conn T. 2012. Use of phosphosphatidylcholine to improve the function of turkey semen stored at 4°C for 24hours. Poult Sci. 91(8):1990-1996. ISSN: 1525-3171. https://doi.org/10.3382/ps.2011-02028
MASOUDI R, Sharafi M, Shahneh AZ, Khodaei-Motlagh M. 2019. Effects of reduced glutathione on the quality of rooster sperm during cryopreservation. Theriogenology. 1(128):149-155. ISSN: 1879-3231. https://doi.org/10.1016/j.theriogenology.2019.01.016
MATSUZAKI M, Sasanami T. 2022. Sperm Motility Regulation in Male and Female Bird Genital Tracts. J Poult Sci. 59(1):1-7. ISSN: 1346-7395.
https://doi.org/10.2141/jpsa.0200105
NAJAFI A, Daghigh KH, Hamishehkar H. 2021. Does alpha-lipoic acid-loaded nanostructured lipid carriers improve post-thawed sperm quality and ameliorate apoptosis-related genes of rooster sperm? Poult Sci. 100(1):357-365. ISSN: 1525-3171. https://doi.org/10.1016/j.psj.2020.10.007
NAKAMURA Y. Avian Biotechnology. 2017. Adv Exp Med Biol. 1001:187-214. ISSN: 2214-8019. https://doi.org/10.1007/978-981-10-3975-1_12
NGUYEN TMD, Seigneurin F, Froment P, Combarnous Y, Blesbois E. 2015. The 5’-AMP-
Activated Protein Kinase (AMPK) Is Involved in the Augmentation of Antioxidant Defenses in Cryopreserved Chicken Sperm. PLoS One. 10(7):e0134420. ISSN: 1932-6203. https://doi.org/10.1371/journal.pone.0134420
NGUYEN QT, Wallner U, Schmicke M, Waberski D, Henning H. 2016. Energy metabolic state in hypothermically stored boar spermatozoa using a revised protocol for efficient ATP extraction. Biol Open. 5 (11):1743-1751. ISSN: 2046-6390.
https://doi.org/10.1242/bio.017954
OLUBOWALE S, Greyling JPC, De Witt FH, Hugo A, Raito MB. 2014. Effects of dietary lipid sources on the semen quality of hy-line silver-brown cockerels. J Anim Plant Sci. 24(4):991-997. ISSN: 1018-7081. http://www.thejaps.org.pk/docs/v-24-4/03.pdf
RESTREPO G, Varela E, Usuga A. 2016. Evaluación de la calidad espermática epididimal en hipopótamos Hippopotamus amphibius (Artiodactyla: hippopotamidae) ubicados en el magdalena medio, Colombia. Ac Zool Mex. 32(2):158-167. ISSN: 2448-8445. https://www.scielo.org.mx/pdf/azm/v32n2/0065-1737-azm-32-02-00158.pdf
ROMO S, López D, Ledesma N, Gutiérrez C, Quintana A, Rangel L. 2022. Comparación en la calidad de huevos obtenidos en un sistema de producción en corrales al aire libre y los producidos en un sistema de jaula. Rev Mex Cien Pec. 13(1):32-42. ISSN: 2448-6698. https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5300/4696
SAJJADI SH, Ahmadzadeh H, Goharshadi EK. 2019. Enhanced electrophoretic separation of proteins by tethered SiO2 nanoparticles in an SDS-polyacrylamide gel network. Analyst. 145(2):415-423. ISSN: 1364-5528.
https://pubs.rsc.org/en/content/articlelanding/2020/an/c9an01759c
SASANAMI TM. 2013. Sperm storage in the female reproductive tract in birds. J Reprod Dev. 59(4):334-8. ISSN: 1348-4400. https://doi.org/10.1262/jrd.2013-038
SEDAGHAT B, Hajjaran H, Sadjjadi FS, Heidari S, Sadjjadi SM. 2021. Proteomic 1089-8646 through optimizing protein extraction. BMC Res Notes. 14(1):22. ISSN: 1756-0500. https://doi.org/10.1186/s13104-020-05433-3
SETIAWAN R, Priyadarshana C, Miyazaki H, Tajima A, Asano A. 2021. Functional difference of ATP-generating pathways in rooster sperm (Gallus gallus domesticus). Anim Reprod Sci. 233:106843. 233. ISSN: 1873-2232.
https://doi.org/10.1016/j.anireprosci.2021.106843
ZHONG TY, Ling L, Feng Z, Zhen-He Z, Maxwell H, Ning Y, Zhuo-Cheng H. 2020. The transcriptome landscapes of ovary and three oviduct segments during chicken (Gallus gallus) egg formation. Genomics. 112(1):243-251. ISSN: 1089-8646.
