Study on erythrocyte osmotic fragility in apparently healthy cholistani camels reared under intensive farming system

Authors

Keywords:

erythrocytic fragility, one-humped camels, hemolysis, hematology, gender

Abstract

The present study is the first of its kind conducted with an aim to assess the level of Erythrocyte Osmotic Fragility as affected by various factors (varying levels of temperature and pH, age and gender) in apparently healthy Cholistani breed of one-humped camels (n=27) being reared under intensive farming system. Blood was aseptically collected and assessed for %age hemolysis (H%) as per prescribed protocols. Results revealed that the H% decreased with increasing pH and with increasing temperatures, and values within reference range were noticed at the pH of 7.4 and 8.5, and at the temperatures of 37°C and 50°C ranging from 0.4±0.1% to 0.9±0.1%, at NaCl concentration of 0.85g/L. Regarding age, adult camels had significantly (P≤0.05) higher H% (1.0±0.2%) as compared to that in young camels (0.68±0.01%) at the NaCl concentration of 0.85g/L. Gender, however, had a non-significant (P≥0.05) effect on H% for male and female camels. This preliminary study furnishes an insight into physiological adaptations of one-humped camel/camel blood in differing environment and will enable to attain maximum productivity in times of stress through appropriate management and feeding practices. We recommend using EOF as a routine hematological attribute and also recommend further studies on H% in pathological conditions of camels.

http://dx.doi.org/10.21929/abavet2023.24                       

e2023-13

https://www.youtube.com/watch?v=dLgMQBEPvvw

 

References

ABDALMULA A, Benashour F, Shmela M, Alnagar F, Abograra I, Buker A. 2019. Blood profile in normal one humped dromedary (Camelus dromedarius) camels in Libya. Part 3: Effect of sex variation on biochemical and haematological blood profile. International Journal of Science and Basic Applied Research. 48:9-24. ISSN: 2395-3446.

https://www.gssrr.org/index.php/JournalOfBasicAndApplied/article/view/10172/4425

ALI I, Chaudhry MS, Farooq U. 2009. Camel rearing in Cholistan desert of Pakistan. Pakistan Veterinary Journal. 29: 85-92. ISSN:0253-8318. http://www.pvj.com.pk/pdf-files/29_2/85-92.pdf

AMIN ASA, Abdoun KA, Abdelatif A. 2012. Erythrocyte osmotic fragility curve of male and female camels (Camelus dromedarius). In "Proceedings of the 3rd Conference of the International Society of Camelid Research and Development". Pp. 288-289. https://www.academia.edu/80370070/Effects_of_Vitamin_E_and_Vitamin_C_on_Hydrogen_Peroxide_Induced_Hemolysis_in_Moroccan_Dromedary_Camels_Camelus_Dromedarius_

AMIN A, Abdoun KA, Abdelatif AM. 2007. Seasonal variation in blood constituents of one-humped camel (Camelus dromedarius). Pakistan Journal of Biological Sciences 10: 1250-1256. https://scialert.net/qredirect.php?doi=pjbs.2007.1250.1256&linkid=pdf

AN X, Mohandas N. 2011. Erythroblastic islands, terminal erythroid differentiation and reticulocyte maturation. International Journal of Hematology 93: 139-143. ISSN :2578-501X. https://link.springer.com/content/pdf/10.1007/s12185-011-0779-x.pdf?pdf=button

AYRES MCC, Birgel EH, Birgel Jr E. 2014. Influence of age, sex and breed lines of Zebu nellore cattle on the erythrocytes osmotic fragility, raised in São Paulo, Brazil. International Journal of Applied Science and Technology 4: 70-75. ISSN 2231-3842.

http://www.ijastnet.com/journals/Vol_4_No_5_October_2014/8.pdf

BALLAS SK, Clark MR, Mohandas N, Colfer H, Caswell M, Bergren M, Perkins H, Shohet S. 1984. Red cell membrane and cation deficiency in Rh null syndrome. Blood 63:1046-1055. ISSN 0006-4971. https://doi.org/10.1182/blood.V63.5.1046.1046

BELLO TK, Ayo JO, Oyelowo BB. 2020. Erythrocyte osmotic fragility of pubertal red sokoto goats administered ascorbic acid during the early rainy season. Alexandria Journal for Veterinary Sciences 66: 10-14. ISSN 2536-9520.

https://www.ejmanager.com/mnstemps/31/31-1584908694.pdf?t=1693651439

BOWDLER A, Dougherty R, Bowdler NC. 1981. Age as a factor affecting erythrocyte osmotic fragility in males. Gerontology 27: 224-231. ISSN: 1423-0003. https://sci-hub.se/https://doi.org/10.1159/000212477

DINI B, Dolfi C, Santucci V, Cavallini G, Donati A, Gori Z, Maccheroni M, Bergamini E. 2001. Effects of ageing and increased haemolysis on the levels of dolichol in rat spleen. Experimental Gerontology 37: 99-105. ISSN 0531-5565.

https://sci-hub.se/https://doi.org/10.1016/S0531-5565(01)00156-5

FAROOQ U, Samad H, Khurshid A, Sajjad S. 2011. Normal reference hematological values of one humped camels (Camelus dromedarius) kept in Cholistan desert. Journal of Animal and Plant Science. 21: 157-160. ISSN1018-7081.

http://www.thejaps.org.pk/docs/21-2/11-117.pdf

FAROOQ U, Samad H, Sher F, Asim M, Khan MA. 2010. Cholistan and Cholistani breed of cattle. Pakistan Veterinary Journal. 30: 2074-7764. ISSN 0253-8318.

http://www.pvj.com.pk/pdf-files/30_2/126-130%20_9117_.pdf

FAULKNER WR, King JW. 1970. Manual of clinical laboratory procedures. Chemical Leather Company, UK. https://cir.nii.ac.jp/crid/1130282269188352000

FERREIRA RF, de Mello Figueiredo Cerqueira A, Pereira AM, Guimarães CM, de Sa AG, da Silva Abreu F, Massard CL, Almosny NP. 2007. Anaplasma platys diagnosis in dogs: comparison between morphological and molecular tests. International Journal of Applied Research in Veterinary Medicine. 5:113. ISSN 1542-2666.

https://bjvm.org.br/BJVM/article/view/478/361

GHOKE S, Jadhav K, Thorat K. 2013. Assessing the osmotic fragility of erythrocytes of rural and semiurban camels (Camelus dromedarius). Camel: An international Journal of Veterinary Sciences. 1: 75-78. ISSN: 2319-9660.

https://www.indianjournals.com/ijor.aspx?target=ijor:cijvs&volume=1&issue=1&article=007

HABIBU B, Yaqub L, Ahmed I, Kawu M, Buhari H, Tauheed M, Isa H. 2013. Erythrocyte osmotic fragility and haematologic parameters of three breeds of 9-week-old broiler chickens. International Journal of Poultry Science. 12: 277-279. ISSN:1682-8356. https://scialert.net/fulltext/?doi=ijps.2013.277.279

IGBOKWE N. 2018. A review of the factors that influence erythrocyte osmotic fragility. Sokoto Journal of Veterinary Sciences. 16: 1-23. ISSN: 2315-6201. https://www.ajol.info/index.php/sokjvs/article/view/183022

IGBOKWE N, Ojo N, Igbokwe I. 2016. Effects of sex and age on the osmotic stability of Sahel goat erythrocytes. Comparative Clinical Pathology. 25:15-22. ISSN 1618-5641. https://link.springer.com/content/pdf/10.1007/s00580-015-2130-z.pdf

KEITT AS, Smith TW, Jandl JH. 1966. Red-cell pseudomosaicism in congenital methemoglobinemia. New England Journal of Medicine. 275(8): 397-405. ISSN: 0028-4793.

https://www.nejm.org/doi/full/10.1056/NEJM196608252750801

LEKTIB I, Bargaâ R, Chakir Y, Belhouari A, Hammoumi A, El Khasmi M. 2016. Study of incubation conditions for erythrocytes osmotic fragility testing in dromedary camel (Camelus dromedarius). International Journal of Research in Environmental Science. 2: 22-32. ISSN 2454-9444. https://www.arcjournals.org/international-journal-of-research-in-environmental-science/volume-2-issue-2/4

LIVESTOCK & Dairy Development Department. 2018. First Real Time (Door to Door) Livestock Census. (P. Livestock & Dairy Development Department, ed.). Pp. 30. L&DD, Punjab, Lahore, Pakistan. https://livestock.punjab.gov.pk/system/files/ADP2019-20_0.pdf

MIJARES A, Vivas J, Abad C, Betancourt M, Piñero S, Proverbio F, Marín R, Portillo R. 2010. Trypanosoma evansi: Effect of experimental infection on the osmotic fragility, lipid peroxidation and calcium-ATPase activity of rat red blood cells. Experimental Parasitology. 124: 301-305. ISSN: 0014-4894.

https://sci-hub.se/https://doi.org/10.1016/j.exppara.2009.11.002

OGBUAGU NE, Aluwong T, Ayo JO, Sumanu VO. 2018. Effect of fisetin and probiotic supplementation on erythrocyte osmotic fragility, malondialdehyde concentration and superoxide dismutase activity in broiler chickens exposed to heat stress. Journal of Veterinary Medical Science. 1: 477-481. ISSN: 0916-7250.

https://www.jstage.jst.go.jp/article/jvms/80/12/80_18-0477/_pdf/-char/ja

OYEWALE JO, Olayerni F, Rahman SA. 1998. Blood characteristics of the Nigerian local duck (Anas platyrhynchos) I. Red blood cell characteristics. Veterinarski Arhiv. 68: 199-204. ISSN 0372-5480. http://www-staro.vef.unizg.hr/vetarhiv/papers/68-6/oyewale.htm

OYWALE J, Dzenda T, Yaqub L, Akanbi D, Ayo J, Oyewele O, Dare T. 2011. Alterations in the osmotic fragility of camel and donkey erythrocytes caused by temperature, pH and blood storage. Veterinarski Arhiv. 81(4):459-470. ISSN 0372-5480.

https://hrcak.srce.hr/file/105582

PATI S, Panda SK, Behera P, Panda M. 2017. Assessment of erythrocyte osmotic fragility in cattle due to haemoprotozoan diseases. International Journal of Science, Environment and Technology. 6: 1560-1568. ISSN: 1735-1472.

https://www.ijset.net/journal/1719.pdf

PERK K. 1966. Osmotic hemolysis of the camel’s erythrocytes. I.A microcinematographic study. Journal of Experimental Zoology. 163(3):241-246. ISSN 2471-5646.

https://onlinelibrary.wiley.com/doi/abs/10.1002/jez.1401630303?casa_token=fM8TsxFRXt4AAAAA:c9JMKcVYrXh9gBUICNF9u3C_K4hN-EBmx0AIvfC7TjWybDddPdwu4iAV5qn9pHAmZnX6yM1sxSZWzpfCoA

PODER TG, Nonkani WG, Leponkouo ÉT. 2015. Blood warming and hemolysis: a systematic review with meta-analysis. Transfusion Medicine Reviews. 29: 172-180. https://sci-hub.se/https://doi.org/10.1016/j.tmrv.2015.03.002

SCHROTER RC, Filali RZ, Brain APR, Jaffrey PK, Robertshaw D. 1990. Influence of dehydration and watering on camel red blood cell size: A scanningelectron microscopic study. Respiration Physiology. 81(3):381-390. ISSN 1569-9048.

https://sci-hub.se/https://doi.org/10.1016/0034-5687(90)90118-I

SEEMAN P, Kwant W, Sauks T, Argent W. 1969. Membrane expansion of intact erythrocytes by anesthetics. Biochimica et Biophysica Acta (BBA)-Biomembranes. 183: 490-498. ISSN:00052736, 18792642.

https://sci-hub.se/https://doi.org/10.1016/0005-2736(69)90163-1

SHARMA B, Rai DK, Rai PK, Rizvi S, Watal G. 2010. Determination of erythrocyte fragility as a marker of pesticide-induced membrane oxidative damage. In "Advanced Protocols in Oxidative Stress II", pp. 123-128. Springer.

https://link.springer.com/protocol/10.1007/978-1-60761-411-1_8

WALLS R, Kumar KS, Hochstein P. 1976. Aging of human erythrocytes: Differential sensitivity of young and old erythrocytes to hemolysis induced by peroxide in the presence of thyroxine. Archives of Biochemistry and Biophysics. 174: 463-468. ISSN: 0003-9861. https://sci-hub.se/https://doi.org/10.1016/0003-9861(76)90374-X

YAGIL R, Luchtenstein C. Meyerstein N. 1976. Hemoconcentration and erythrocyte fragility in chickens exposed to heat and dehydration. American Journal of Veterinary Research. 37:103-6. ISSN: 0002-9645. https://europepmc.org/article/med/1247191

YAGIL R, Sod-Moriah UA, Meyerstien N. 1974. Dehydration and camel blood.3. Osmotic fragility, specific gravity and osmolality. American Journal of Physiology-Legacy Content, 226(2):305-308. ISSN 0002-9513.

https://sci-hub.se/https://doi.org/10.1152/ajplegacy.1974.226.2.305

YAQUB LS, Mshelia WP, Ayo JO. 2014. Erythrocyte osmotic fragility and hematological responses of horses administered ascorbic acid and exposed to road transportation. Journal of Equine Veterinary Science. 34: 1324-1328. ISSN 15427412, 07370806. https://sci-hub.se/https://doi.org/10.1016/j.jevs.2014.09.015

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2023-12-11

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