Efeito dos antioxidantes Trolox e Crocina na criopreservação do sêmen de ovelhas Pelibuey
Palavras-chave:
espermaResumo
O objetivo do estudo foi avaliar o efeito dos antioxidantes Trolox e Crocina no diluente de congelamento na criopreservação do sêmen de ovelha Pelibuey. Os ejaculados obtidos foram misturados, divididos e congelados em 3 tratamentos: T1: Trolox 1mM; T2: Crocina 1 mM; TT: Testemunha (sem antioxidante). Às 0 horas (degelo) e 6 horas a 37 °C, foram analisadas motilidade total (MT) e progressiva (MP), viabilidade espermática, atividade mitocondrial, integridade dos acrossomas e membrana da cauda (Anfitrião) Os dados foram analisados com ANOVA para delineamento inteiramente casualizado e teste de Tukey para comparação de médias. Em nenhuma das duas horas foram encontradas diferenças significativas nos tratamentos (P>0,05). Entretanto, de 0 a 6h, a MT se comportou de maneira semelhante em T1 e T2, mas não em TT (P<0,01), pelo contrário, a integridade dos acrossomas nos três tratamentos foi semelhante (P>0,05). MP, viabilidade, atividade mitocondrial e hospedeiro diminuíram ao longo do tempo de maneira semelhante nos três tratamentos. Os antioxidantes Trolox e Crocina a 1mM no diluente de congelamento permitem manter a MT e a integridade dos acrossomas por 6 h de incubação a 37 °C.
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