Efecto descapacitante de proteínas oviductales en espermatozoides de gallo in vitro


Palabras clave:

semen, adenosin trifosfato, glutation reducido, malondialdehido, oxidación


En las aves, no se ha descrito totalmente el proceso de maduración espermática. El objetivo del estudio fue determinar parámetros in vitro, del efecto descapacitante de las proteínas oviductales, en espermatozoides de gallo. Alícuotas con espermatozoides, fueron incubadas in vitro para inducir estados metabólicos de capacitación, descapacitación y reacción acrosomal para determinar porcentajes de espermatozoides vivos, su movilidad y concentraciones de Malondialdehído, Glutation reducido y Adenosin tri fosfato, como parámetros del estado metabólico de los espermatozoides. Los resultados mostraron porcentajes de movilidad y espermatozoides vivos en semen fresco y capacitado similares (P>0.05), pero mayores (P<0.05) a los determinados en espermatozoides con reacción acrosomal. Los nmol/ml de MDA en semen fresco (1.59) fue mayor (P<0.005) que en semen capacitado (1.05), con reacción acrosomal (1.07) y en semen descapacitado (1.05). Los nmol/ml de GSG fueron similares (P>0.05) en semen fresco (72.6) y capacitado (62.04), y entre semen con reacción acrosomal (99.09) y descapacitado (86.07). La mayor concentración de ATP fue en semen capacitado con 69.9 µmol/ml, con concentraciones similares (P<0.05) en semen fresco, con reacción acrosomal y descapacitados. Los parámetros determinados, demostraron que la fracción proteica de la unión útero-vaginal, in vitro produce descapacitación y mantiene la viabilidad espermática.






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