Sobrevivência da Pasteurella multocida isolada de coelhos à lise induzida pelo sistema de complemento
Palavras-chave:
Pasteurelose, inibição do desenvolvimento, bactérias, complexo de ataque à membranaResumo
A pasteurelose é uma doença bacteriana que causa danos econômicos em sistemas de produção de coelhos em todo o mundo e é uma zoonose comprovada em humanos infectados por animais de companhia. O sistema de complemento causa a lise de vários microrganismos patogênicos ao formar o complexo de ataque à membrana. O objetivo do presente trabalho foi avaliar a atividade do complemento do soro humano sobre a capacidade de sobrevivência de um isolado virulento de Pasteurella multocida tipo A. A bactéria foi obtida de um caso clínico de um coelho com pneumonia, por meio de esfregaço nasal para isolamento em cultura pura e identificação por testes bioquímicos. A P. multocida foi desafiada por 30 minutos a 37 °C, soro humano normal e soro humano inativado pelo calor. A lise por ação do complemento teve uma redução significativa na viabilidade do microrganismo em comparação com a cultura desafiada com complemento inativado (P<0,05); no entanto, 51,07 ± 1,70 % conseguiram escapar da atividade lítica do complemento. A presente investigação conclui que o sistema de complemento oferece proteção parcial contra infecções por P. multocida sorotipo A que causam pasteurelose pneumônica em coelhos.
http://dx.doi.org/10.21929/abavet2022.231
e2022-45
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