Partial resistance of Pasteurella multocida isolated from rabbits to lysis induced by the complement system
Keywords:
Pasteurellosis, developmental inhibition, bacteria, membrane attack complexAbstract
Pasteurellosis is one of the most serious bacterial diseases of rabbits, causes considerable economic damage in production systems worldwide and is a widely proven zoonosis with cases in humans infected by companion animals. The activated complement system causes lysis of various pathogenic microorganisms by the formation of the membrane attack complex. The objective of the present work was to evaluate the survivability of a virulent isolate of Pasteurella multocida type A to human serum complement activity. A female rabbit was referred as a clinical case of pneumonic pasteurellosis, a nasal swab was performed for isolation and identification. P. multocida was isolated in pure culture, characterized by standard biochemical identification tests, then challenged for 30 min at 37°C with normal serum and heat-inactivated serum. Lysis by complement action had a significant reduction in the viability of the microorganism with respect to the culture challenged with inactivated complement (p< 0.05), however, a significant percentage 51.07 ± 1.70 % managed to evade complement lytic activity. In the context of health, it is concluded that the complement system offers partial protection against infections by P. multocida serotype A causing pneumonic pasteurellosis in rabbits.
http://dx.doi.org/10.21929/abavet2022.231
e2022-45
References
ABREU F, Rodríguez-Lucas C, Rodicio MR, Vela AI, Fernández-Garayzábal JF, Leiva, PS, Fernández J. 2018. Human Pasteurella multocida infection with likely zoonotic transmission from a pet dog, Spain. Emerging Infectious Diseases. 24(6):1145. ISSN: 1080-6059. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004854/
AKTORIES K, Orth JH, Adler B. 2012. Pasteurella multocida. Current Topics of Microbiology and Immunology. 361:1. ISSN: 0070-217X.
http://www.ncbi.nlm.nih.gov/pubmed/23230602
BAJIC G, Degn SE, Thiel S, Andersen GR. 2015. Complement activation, regulation, and molecular basis for complement‐related diseases. Journal of the European Molecular Biology Organization. 34(22):2735-2757. ISSN: 0261-4189.
https://doi.org/10.15252/embj.201591881
BAUD D, Bizzini A, Jaton K, Achtari C, Prod'hom G, Greub G. 2012. Pasteurella multocida zoonotic ascending infection: an unusual cause of tubo-ovarian abscess. Vector-Borne and Zoonotic Diseases. 12(1):84-85. ISSN: 1530-3667.
https://doi.org/10.1089/vbz.2011.0610
BOURÉLY C, Cazeau G, Jouy E, Haenni M, Madec JY, Jarrige N, Gay E. 2019. Antimicrobial resistance of Pasteurella multocida isolated from diseased food-producing animals and pets. Veterinary Microbiology. 235:280-284. ISSN: 0378-1135.
https://doi.org/10.1016/j.vetmic.2019.07.017
CARROLL MC, Isenman DE. 2012. Regulation of humoral immunity by complement. Immunity. 37(2):199-207. ISSN: 1074-7613.
https://doi.org/10.1016/j.immuni.2012.08.002
D’AMICO F, Casalino G, Bozzo G, Camarda A, Lombardi R, Dimuccio MM, Circella E. 2022. Spreading of Pasteurella multocida Infection in a Pet Rabbit Breeding and Possible Implications on Healed Bunnies. Veterinary Sciences. 9(6):301. ISSN: 2306-7381. https://doi.org/10.3390/vetsci9060301
DZIVA F, Muhairwa AP, Bisgaard M, Christensen H. 2008. Diagnostic and typing options for investigating diseases associated with Pasteurella multocida. Veterinary Microbiology. 128(1-2):1-22. ISSN: 0378-1135.
https://doi.org/10.1016/j.vetmic.2007.10.018
EL‐SHEIKH SM, Youssef FM, Mohamed HI, El‐Saber Batiha G, Albrakati A, Galal AA. 2021. Efficacy of grape seed hydro‐alcoholic extract in the treatment of experimentally Pasteurella multocida infected rabbits. Veterinary Medicine and Science. 7(3):923-934. ISSN: 2053-1095. https://doi.org/10.1002/vms3.446
ELAZAB ST, Schrunk DE, Griffith RW, Ensley SM, Dell'Anna G, Mullin K, Hsu WH. 2018. Pharmacokinetics of cefquinome in healthy and Pasteurella multocida‐infected rabbits. Journal of veterinary pharmacology and therapeutics. 41(3):374-377. ISSN: 1365-2885. https://doi.org/10.1111/jvp.12489
FERREIRA TSP, Moreno LZ, Felizardo MR, de Gobbi DDS, Filsner PHdLN, de Moura Gomes VT, Moreno AM. 2016. Pheno-and genotypic characterization of Pasteurella multocida isolated from cats, dogs and rabbits from Brazil. Comparative Immunology, Microbiology and Infectious Diseases. 45:48-52. ISSN: 2356-6140.
https://doi.org/10.1016/j.cimid.2016.02.004
GRAPHPAD Prism. 2012. GraphPad Software®. version 6.0.0 for Windows, San Diego, California USA. www.graphpad.com
GUAN L, Zhang L, Xue Y, Yang J, Zhao Z. 2020. Molecular pathogenesis of the hyaluronic acid capsule of Pasteurella multocida. Microbial pathogenesis. 149:104380. ISSN: 0882-4010. https://doi.org/10.1016/j.micpath.2020.104380
GUNATHILAKE R, Verma A, Caffery M, Sowden S. 2015. Pasteurella multocida peritonitis after cat scratch in a patient with cirrhotic ascites. Infectious Disease Reports. 7(2):5937. ISSN: 2036-7449. https://doi.org/10.4081/idr.2015.5937
HARPER M, Boyce JD, Adler B. 2012. The Key Surface Components of Pasteurella multocida: Capsule and Lipopolysaccharide. In: Aktories K, Orth J, Adler B. Pasteurella multocida. Current Topics in Microbiology and Immunology. 361. Springer, Berlin, Heidelberg. ISBN: 9783642310164. https://doi.org/10.1007/82_2012_202
HEY P, Gow P, Torresi J, Testro A. 2012. Cirrhosis, cellulitis and cats: a ‘purrfect’combination for life-threatening spontaneous bacterial peritonitis from Pasteurella multocida. Case Reports. British Medical Journal. 2012:007397. ISSN: 1757-790X. http://dx.doi.org/10.1136/bcr-2012-007397
JAMALI H, Rezagholipour M, Fallah S, Dadrasnia A, Chelliah S, Velappan RD, Ismail S. 2014. Prevalence, characterization and antibiotic resistance of Pasteurella multocida isolated from bovine respiratory infection. The Veterinary Journal. 202(2):381-383. ISSN: 1090-0233. https://doi.org/10.1016/j.tvjl.2014.07.024
KONEMAN EW, Allen S. 2008. Koneman. Diagnostico Microbiologico/Microbiological diagnosis: Texto Y Atlas En Color/Text and Color Atlas: Ed. médica panamericana. ISBN: 9500608952.
KUBATZKY KF. 2012. Pasteurella multocida and immune cells. Pasteurella multocida. 53-72. ISBN: 9783642310171. https://link.springer.com/book/10.1007/978-3-642-31017-1
LI N, Feng T, Wang Y, Li P, Yin Y, Zhao Z, He F. 2021. A single point mutation in the hyaC gene affects Pasteurella multocida serovar A capsule production and virulence. Microbial pathogenesis. 159:105145. ISSN: 0882-4010.
https://doi.org/10.1016/j.micpath.2021.105145
LIN PH, Bush RL, Zhou W, Peden EK, Lumsden AB. 2006. Endovascular treatment of traumatic thoracic aortic injury—should this be the new standard of treatment? Journal of vascular surgery. 43(2):22-29. ISSN: 1097-6809.
https://doi.org/10.1016/j.jvs.2005.10.068
MASSACCI FR, Magistrali CF, Cucco L, Curcio L, Bano L, Mangili P, Christensen H. 2018. Characterization of Pasteurella multocida involved in rabbit infections. Veterinary Microbiology. 213:66-72. ISSN: 1873-2542. https://doi.org/10.1016/j.vetmic.2017.11.023
MATHERN DR, Heeger PS. 2015. Molecules great and small: the complement system. Clinical Journal of the American Society of Nephrology. 10(9):1636-1650. ISSN: 1555-905X. https://doi.org/10.2215/cjn.06230614
MORENO-TORRES A, Malvido-Jiménez IR, de la Peña-Moctezuma A, Castillo-Sánchez LO, Fraga TR, Barbosa AS, Sahagún-Ruiz A. 2019. Culture-attenuated pathogenic Leptospira lose the ability to survive to complement-mediated-killing due to lower expression of factor H binding proteins. Microbes and Infection. 21(8-9):377-385. ISSN: 1769-714X. https://doi.org/10.1016/j.micinf.2019.03.001
NICHOLSON LB. 2016. The immune system. Essays in biochemistry. 60(3):275-301. ISSN: 1744-1358. https://doi.org/10.1042/ebc20160017
PALÓCZ O, Gál J, Clayton P, Dinya Z, Somogyi Z, Juhász C, Csikó G. 2014. Alternative treatment of serious and mild Pasteurella multocida infection in New Zealand White rabbits. BMC Veterinary Research. 10(1):1-7. ISSN: 1746-6148.
https://doi.org/10.1186/s12917-014-0276-6
PANNA SN, Nazir KNH, Rahman MB, Ahamed S, Saroare MG, Chakma S, Majumder UH. 2015. Isolation and molecular detection of Pasteurella multocida Type A from naturally infected chickens, and their histopathological evaluation in artificially infected chickens in Bangladesh. Journal of Advanced Veterinary and Animal Research. 2(3):338-345. ISSN: 2311-7710. http://dx.doi.org/10.5455/javar.2015.b104
PER H, Kumandaş S, Gümüş H, Öztürk MK, Çoşkun A. 2010. Meningitis and subgaleal, subdural, epidural empyema due to Pasteurella multocida. The Journal of emergency medicine. 39(1):35-38. ISSN:0736-4679.
https://doi.org/10.1016/j.jemermed.2008.04.008
QUESADA SP, Paschoal JAR, Reyes FGR. 2013. Considerations on the aquaculture development and on the use of veterinary drugs: special issue for fluoroquinolones-a review. Journal of Food Science. 78(9):R1321-R1333. ISSN: 1750-3841.
https://doi.org/10.1111/1750-3841.12222
REGISTER KB, Brockmeier SL. 2019. Pasteurellosis. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW, Zhang J. Diseases of swine 884-897. John Wiley and Sons. Hoboken. ISBN: 9781119350927.
https://doi.org/10.1002/9781119350927.ch57
RICKLIN D, Hajishengallis G, Yang K, Lambris JD. 2010. Complement: a key system for immune surveillance and homeostasis. Nature immunology. 11(9):785-797. ISSN: 1529-2916. https://doi.org/10.1038/ni.1923
ROSELL J, de La Fuente L. 2016. Causes of mortality in breeding rabbits. Preventive Veterinary Medicine. 127:56-63. ISSN:1873-1716.
https://doi.org/10.1016/j.prevetmed.2016.03.014
SAHAGUN-RUIZ A, Granados Martínez AP, Breda LC, Fraga TR, Castiblanco Valencia MM, Barbosa AS, Isaac L. 2014. Pasteurella pneumotropica evades the human complement system by acquisition of the complement regulators factor H and C4BP. PloS One. 9(10):e111194. ISSN: 1932-6203.
https://doi.org/10.1371/journal.pone.0111194
SORIANO-VARGAS E, Vega-Sánchez V, Zamora-Espinosa JL, Acosta-Dibarrat J, Aguilar-Romero F, Negrete-Abascal E. 2012. Identification of Pasteurella multocida capsular types isolated from rabbits and other domestic animals in Mexico with respiratory diseases. Tropical Animal Health Production. 44(5):935-937. ISSN:1573-7438.
https://doi.org/10.1007/s11250-011-9995-x
SOUZA MJ. 2009. Bacterial and Parasitic Zoonoses of Exotic Pets. Veterinary Clinics of North America: Exotic Animal Practice. 12(3):401-415. ISSN: 1558-4232.
https://doi.org/10.1016/j.cvex.2009.06.003
World Organization for Animal Healt (WOAH). 2012. Manual of diagnostic tests and vaccines for terrestrial animals. Infection Bursal Disease. 12:549-565. ISBN: 9290446226. https://doi.org/10.1017/S0031182005007699
WILSON BA, Ho M. 2013. Pasteurella multocida: from zoonosis to cellular microbiology. Clinical microbiology reviews. 26(3):631-655. ISSN: 1098-6618.
https://doi.org/10.1128/cmr.00024-13
ZHU W, Fan Z, Qiu R, Chen L, Wei H, Hu B, Wang F. 2020. Characterization of Pasteurella multocida isolates from rabbits in China. Veterinary Microbiology. 244:108649. ISSN: 1873-2542. https://doi.org/10.1016/j.vetmic.2020.108649
