Comparação do uso do refratômetro Brix e do colostrômetro para estimar a qualidade do colostro em vacas leiteiras
Palavras-chave:
colostro, brix, colostrômetro, vacas leiteirasResumo
O colostro é a primeira secreção mamária e é a única maneira pela qual os bezerros adquirem imunidade
passiva. O objetivo deste estudo é determinar a correlação da qualidade do colostro de vacas leiteiras de
alta produção medida pelo colostrômetro e pela refratometria digital de graus Brix (°Bx). Foram colhidas
340 amostras de colostro do mesmo número de vacas e analisadas por colostrômetro e refratometria de
°Bx. Foi realizada uma regressão linear simples e o coeficiente de determinação (R²) foi determinado. Tudo
isso por meio do pacote estatístico SPSS 25.0. A média com o calostrômetro foi de 87,94±110, enquanto
com a refratometria Brix foi de 26,19±18, com um R2 0,706 com um valor de P<0.0001. Em conclusão, as
leituras de refratometria e o calostrômetro são métodos eficazes na determinação de imunoglobulinas. O
refratômetro Brix teve uma correlação aceitável, o que pode ser uma ferramenta fácil para verificar a
qualidade do colostro ao pé da vaca.
http://dx.doi.org/10.21929/abavet2024.5
e2021-82
Referências
BARTENS MC, Drillich M, Rychli K, Iwersen M, Arnholdt T, Meyer L, Klein-Jöbstl D. 2016. Assessment of different methods to estimate bovine colostrum quality on farm. New Zealand Veterinary Journal. 64(5):263-7.
https://doi.org/10.1080/00480169.2016.1184109
BARTIER AL, Windeyer MC, Doepel L. 2015. Evaluation of on-farm tools for colostrum quality measurement. Journal of Dairy Science. 98(3):1878-84.
https://doi.org/10.3168/jds.2014-8415
BAUMRUCKER CR, Burkett AM, Magliaro-Macrina AL, Dechow CD. 2010. Colostrogenesis: Mass transfer of immunoglobulin G1 into colostrum. Journal of dairy science. 93(7):3031-8.
https://doi.org/10.3168/jds.2009-2963
BIELMANN V, Gillan J, Perkins NR, Skidmore AL, Godden S, Leslie KE. 2010. An evaluation of Brix refractometry instruments for measurement of colostrum quality in dairy cattle. Journal of Dairy Science. 93(8):3713-21.
https://doi.org/10.3168/jds.2009-2943
BUCZINSKI S, Vandeweerd JM. 2016. Diagnostic accuracy of refractometry for assessing bovine colostrum quality: A systematic review and meta-analysis. Journal of Dairy Science. 99(9):7381-94. https://doi.org/10.3168/jds.2016-10955
CHIGERWE M, Hagey JV. 2014. Refractometer assessment of colostral and serum IgG and milk total solids concentrations in dairy cattle. BMC Veterinary Research. 10(1):1-6. https://doi.org/10.1186/s12917-014-0178-7
DRIKIC M, Windeyer C, Olsen S, Fu Y, Doepel L, De Buck J. 2018. Determining the IgG concentrations in bovine colostrum and calf sera with a novel enzymatic assay. Journal of Animal Science and Biotechnology. 9(1):1-9.
https://doi.org/10.1186/s40104-018-0287-4
FASS. 2010. Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching, 3rd ed.; Federation Animal Science Society: Champaing, IL, USA; p. 177. https://www.fass.org/images/science-policy/Ag_Guide_3rd_ed.pdf
FLEENOR WA, Stott GH. 1980. Hydrometer test for estimation of immunoglobulin concentration in bovine colostrum. Journal of Dairy Science. 63(6):973-7. https://doi.org/10.3168/jds.S0022-0302(80)83034-7
GAMSJÄGER L, Elsohaby I, Pearson JM, Levy M, Pajor EA, Haines DM, Windeyer MC. 2020. Assessment of Brix refractometry to estimate immunoglobulin G concentration in beef cow colostrum. Journal of Veterinary Internal Medicine. 34(4):1662-73. https://doi.org/10.1111/jvim.15805
GELSINGER SL, Smith AM, Jones CM, Heinrichs AJ. 2015. Comparison of radial immunodiffusion and ELISA for quantification of bovine immunoglobulin G in colostrum and plasma. Journal of Dairy Science. 98(6):4084-9. https://doi.org/10.3168/jds.2014-8491
GODDEN S. 2008. Colostrum management for dairy calves. Veterinary Clinics of North America: Food Animal Practice. 24(1):19-39.
https://doi.org/10.1016/j.cvfa.2007.10.005
HASSAN AA, Ganz S, Schneider F, Wehrend A, Khan IU, Failing K, Bülte M, Abdulmawjood A. 2020. Quantitative assessment of German Holstein dairy cattle colostrum and impact of thermal treatment on quality of colostrum viscosity and immunoglobulins. BMC Research Notes. 13(1):1-6. https://doi.org/10.1186/s13104-020-05019-z
JOHNSEN JF, Sørby J, Mejdell CM, Sogstad ÅM, Nødtvedt A, Holmøy IH. 2019. Indirect quantification of IgG using a digital refractometer, and factors associated with colostrum quality in Norwegian Red Cattle. Acta Veterinaria Scandinavica. 61(1):1-9. https://doi.org/10.1186/s13028-019-0494-9
MECHOR GD, Gröhn YT, Van Saun RJ. 1991. Effect of temperature on colostrometer readings for estimation of immunoglobulin concentration in bovine colostrum. Journal of Dairy Science. 74(11):3940-3. https://doi.org/10.3168/jds.S0022-0302(91)78587-1
MENON, P Ramesh, Rakesh Lodha, SK Kabra. 2010. Bovine colostrum in pediatric respiratory diseases: a systematic review. The Indian Journal of Pediatrics. 77: 108-09. https://doi.org/10.1007/s12098-009-0257-0
MORIN DE, Constable PD, Maunsell FP, McCoy GC. 2001. Factors associated with colostral specific gravity in dairy cows. Journal of Dairy Science. 84(4):937-43. https://doi.org/10.3168/jds.S0022-0302(01)74551-1
MORRILL KM, Robertson KE, Spring MM, Robinson AL, Tyler HD. 2015. Validating a refractometer to evaluate immunoglobulin G concentration in Jersey colostrum and the effect of multiple freeze–thaw cycles on evaluating colostrum quality. Journal of Dairy Science. 98(1):595-601. https://doi.org/10.3168/jds.2014-8730
NAM. 2010. Guide for the Care and Use of Laboratory Animals, 1st ed.; National Academy of Medicine: Harlan, Mexico City, Mexico.
https://www.ncbi.nlm.nih.gov/books/NBK54050/pdf/Bookshelf_NBK54050.pdf
NATIONAL ANIMAL HEALTH MONITORING SYSTEM (US). Dairy 2007: Heifer Calf Health and Management Practices on US Dairy Operations, 2007. US Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, National Animal Health Monitoring System; 2010.
QUIGLEY JD, Lago A, Chapman C, Erickson P, Polo J. 2013. Evaluation of the Brix refractometer to estimate immunoglobulin G concentration in bovine colostrum. Journal of Dairy Science. 96(2):1148-55. https://doi.org/10.3168/jds.2012-5823
STELWAGEN K, Carpenter E, Haigh B, Hodgkinson A, Wheeler TT. 2009. Immune components of bovine colostrum and milk. Journal of Animal Science. 87(suppl_13):3-9. https://doi.org/10.2527/jas.2008-1377