Evolução da aspiração folicular para a produção in vitro de embriões em ruminantes
Palavras-chave:
embriões produzidos in vitro, aspiração folicular ovariana, ruminantesResumo
Desde o nascimento da primeira prole gerada a partir da produção in vitro de embriões (PIV) em 1982, os avanços nessa tecnologia não pararam. A PIV foi tão bem-sucedida que foi adaptada a uma variedade de espécies, tanto produtivas quanto selvagens. Sem dúvida, o maior sucesso da PIV foi em bovinos, de acordo com os dados relatados pela Sociedade Internacional de Tecnologia de Embriões (IETS) em 2020. Esse sucesso se deve em grande parte à evolução dos sistemas de cultura in vitro e à coleta eficiente e minimamente invasiva de oócitos de doadoras vivas. Nas últimas três décadas, os avanços na fisiologia ovariana, no desenvolvimento da ultrassonografia, nos tipos de agulha, na pressão de aspiração e nos genótipos utilizados permitiram que a aspiração folicular ovariana fosse uma técnica que pode ser desenvolvida em nível de campo, com resultados cada vez melhores e sem afetar o bem-estar das doadoras. Esta revisão descreverá a evolução da técnica de aspiração folicular em ruminantes, com ênfase em suas variantes e novas estratégias para melhorar a qualidade dos oócitos.
http://dx.doi.org/10.21929/abavet2023.110
e2023-110
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