Evolution of follicular aspiration for in vitro embryo production in ruminants
Keywords:
In vitro produced embryos, ovarian follicular aspiration, ruminantsAbstract
Since the birth of the first offspring generated from in vitro embryo production (IVP) in 1982, advances in this technology have not stopped. PIV has been so successful that it has been adapted to various species, both productive and wild. Undoubtedly, the greatest success of PIV has been in cattle, according to data reported by the International Embryo Technology Society (IETS). This success is largely due to the evolution of in vitro culture systems and the efficient and minimally invasive collection of oocytes from living donors. In the last 3 decades, advances in ovarian physiology, development of ultrasonography, types of needles, aspiration pressure, used genotypes, have allowed follicular aspiration to be a technique that can be developed at the field level, with results better each time without affecting the well-being of the donors. In this review, the evolution of the follicular aspiration technique in ruminants will be described with emphasis on its variants and new strategies to improve oocyte quality.
http://dx.doi.org/10.21929/abavet2023.110
e2023-110
https://www.youtube.com/watch?v=bhUxhxTiNDc
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