Avaliação do efeito antiparasitário das nanopartículas de prata contra o Trypanosoma cruzi in vitro
Palavras-chave:
Trypanosoma cruzi, Chagas, AgNP, in vitro, prataResumo
A tripanossomíase americana é uma das doenças tropicais negligenciadas globalmente e é causada pelo protozoário hemoflagelado Trypanosoma cruzi (Tc). Atualmente, os medicamentos anti-Tc são limitados e não há vacina disponível. O objetivo do estudo foi determinar os efeitos das nanopartículas de prata (AgNPs) contra tripomastigotas das cepas Sylvio X10/4 (TcI) e Esmeraldo (TcII). As AgNPs foram obtidas pelo método de redução e foi realizada uma caracterização estrutural. A atividade citotóxica das AgNPs (atividade anti-Tc) foi determinada pelo cálculo dos valores da metade da concentração inibitória máxima (IC50) em células HaCaT e parasitas. As AgNPs não apresentaram citotoxicidade contra as células HaCaT nos valores de IC50 testados em parasitas, e a IC50 tripomastigotas calculada foi de 4,05 μg/ml e 6,16 μg/ml para as cepas TcI e TcII, respectivamente. As AgNPs foram mais ativas contra ambas as cepas de Trypanosoma cruzi do que o nifurtimox (400 μg/ml) em ensaios in vitro. Esses resultados estabelecem as bases para uma avaliação mais aprofundada das AgNPs, para investigar seu valor quimioterápico no combate à infecção por T. cruzi em modelos animais.
http://dx.doi.org/10.21929/abavet2023.15
e2022-64
https://www.youtube.com/watch?v=vPcSyYwFCcQ
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