Evaluation of antiparasitic effect of silver nanoparticles against Trypanosoma cruzi in vitro
Keywords:
Trypanosoma cruzi, Chagas, AgNP, in vitro, silverAbstract
American trypanosomiasis is one the globally neglected tropical disease, and is cause by the protozoan haemoflagellate Trypanosoma cruzi (Tc). Currently, the anti-Tc drugs are limited and no vaccine is available. The objective of the study was to determine the effects of silver nanoparticles (AgNPs) against trypomastigotes of the Sylvio X10/4 (TcI) and Esmeraldo (TcII) strains. The AgNPs were obtained by the reduction method and a structural characterization was carried out. The cytotoxic activity of the AgNPs (anti-Tc activity) was determined by calculating the values of half the maximum inhibitory concentration (IC50) in HaCaT cells and parasites. AgNPs showed no-cytotoxicity against HaCaT cells at the IC50 values assayed in parasites, and the calculated IC50 trypomastigotes was 4.05 µg/ml and 6.16 µg/ml for TcI and TcII strains, respectively. AgNPs were more active against both Trypanosoma cruzi strains than nifurtimox (400 µg/ml) in vitro assays. These results lay the groundwork for further evaluation of AgNPs, to investigate its chemotherapeutic value to figh T. cruzi infection in animal models.
http://dx.doi.org/10.21929/abavet2023.15
e2022-64
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