Determination of the quality of semen cryopreserved with soy lecithin or egg yolk, in male goats
Keywords:
Soy lecithin, diluent, goat semenAbstract
The objective was to compare the quality of cryopreserved goat semen with soy lecithin or egg yolk. The semen was collected from male goats (n=4), two commercial diluents AndroMed® (1% soy lecithin, LS); Optidyl® (20% (v/v) Tris-egg yolk; TY), and a citrate-egg yolk-based diluent (CY) were used in fresh semen (SF) and then cooled from 37 to 4 °C for 2 h (refrigerated semen, SR), afterwards straws were filled with semen and frozen in liquid nitrogen at -196 °C (SC). There were no differences (p>0.05) between diluents in the SF in the mass motility (MM; 4.7±0.26), sperm viability (VE; 74.1±1.66) and individual motility (MI; 62.3±4.0). In the same sense, for the SR there was no difference (p>0.05) between diluents with respect to MM (3.83±0.4) and MI (52.1±6.0), however, the VE varied (p<0.05) according to the diluent, observing the lowest viability in LS vs CY and TY (51.0±13.0 vs 71.3±3.0 and 69.0±3.1). Regarding SC the MM, MI and VE obtained better values (p<0.05) with the diluent TY vs LS and CY (2.4±0.5, 32.5±8.3, 41.3±13.0). The results showed a better cryopreservation of goat semen with the diluent Tris-yolk compared to that of soy lecithin.
http://dx.doi.org/10.21929/abavet2021.8
e2020-75.
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