Caracterización de aislamientos de Mannheimia haemolytica y Pasteurella multocida obtenidos de cabritos con problemas respiratorios

Imelda  Martínez-Razo; Erika  Palomares-Resendiz; Francisco  Trigo-Tavera; Luis  Gómez-Nuñez; Francisco  Aguilar-Romero; Efrén  Díaz-Aparicio

Authors

Imelda Martínez-Razo Universidad Nacional Autónoma de México https://orcid.org/0000-0002-9299-7068
Erika Palomares-Resendiz Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias https://orcid.org/0000-0001-5561-338X
Francisco Trigo-Tavera Universidad Nacional Autónoma de México https://orcid.org/0000-0003-1855-2252
Luis Gómez-Nuñez Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias https://orcid.org/0000-0002-3301-1510
Francisco Aguilar-Romero Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias https://orcid.org/0000-0003-1283-0370
Efrén Díaz-Aparicio Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias https://orcid.org/0000-0002-1669-1323

Keywords:

Mannheimia haemolytica, Pasteurella multocida, kids, Mexico

Abstract

The objective of this study was to characterize and determine the presence of Mannheimia haemolytica and Pasteurella multocida isolated from kids with respiratory problems in Mexico. Convenience samplings were carried out in seven states of Mexico, obtaining 1,498 samples of nasal exudate from kids, the samples were inoculated on blood agar plates, and the isolates underwent biochemical tests to determine gender and species, finding 535 isolates, of which 491 were identified as M. haemolytica and 44 as P. multocida. Fifty isolates of M. haemolytica were randomly selected to be characterized with real-time PCR and endpoint PCR, confirming molecular identification in 46 of the 50 isolates. The capsular type and biotype of the P. multocida isolates were determined, finding that 36 isolates corresponded to type A and eight isolates to type D.

https://www.youtube.com/watch?v=FCJCChODYkU

e2023-101

http://dx.doi.org/10.21929/abavet2023.101

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