Caracterización de aislamientos de Mannheimia haemolytica y Pasteurella multocida obtenidos de cabritos con problemas respiratorios
Palabras clave:
Mannheimia haemolytica, Pasteurella multocida, cabritos, MéxicoResumen
El objetivo del presente estudio fue caracterizar y determinar la presencia de Mannheimia haemolytica y Pasteurella multocida aisladas de cabritos con problemas respiratorios en México. Se realizaron muestreos de conveniencia en siete estados de México, obteniéndose 1, 498 muestras de exudado nasal de cabritos, las muestras fueron inoculadas en placas de agar sangre, a los aislamientos se les realizaron pruebas bioquímicas para determinar género y especie, encontrando 535 aislamientos, de los cuales 491 fueron identificados como M. haemolytica y 44 como P. multocida. Se seleccionaron aleatoriamente 50 aislamientos de M. haemolytica para caracterizarlos con PCR tiempo real y PCR punto final, confirmándose la identificación molecular con ambas pruebas en 46 de los 50 aislamientos. A los 44 aislamientos de P. multocida se les determinó el tipo capsular y biotipo, encontrándose que, 36 correspondieron al tipo A y 8 al tipo D.
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