Evaluación del efecto antiparasitario de las nanoparticulas de plata contra Trypanosoma cruzi in vitro
Palabras clave:
Trypanosoma cruzi, Chagas, AgNP, in vitro, plataResumen
La tripanosomiasis Americana es una de las enfermedades tropicales desatendidas a nivel mundial, y es causada por el protozoario hemoflagelado Trypanosoma cruzi (Tc). Actualmente, los fármacos anti-Tc son limitados y no hay vacuna disponible. El objetivo de esta investigación fue evaluar el efecto de las nanopartículas de plata (AgNPs) contra los tripomastigotes de las cepas Sylvio X10/4 (TcI) y Esmeraldo (TcII). Las AgNPs se obtuvieron por el método de reducción y se les realizó una caracterización estructural. La actividad citotóxica de las AgNPs (actividad anti-Tc) se determinó mediante el cálculo de los valores de la mitad de la concentración inhibitoria máxima (IC50) en las células HaCaT y en los parásitos. Las AgNPs no mostraron citotoxicidad contra las células HaCaT en los valores de IC50 probadas en los parásitos, y la IC50 calculada en los tripomastigotes fue de 4.05 µg/ml y de 6.16 µg/ml en las cepas TcI y TcII respectivamente. Las AgNPs fueron más activas contra las dos cepas de Trypanosoma cruzi, que el nifurtimox (400 µg/ml) in vitro. Estos resultados sientan las bases para la evaluación mayor de las AgNPs, por investigar su potencial quimioterapéutico para combatir la infección por T. cruzi en modelos animales.
http://dx.doi.org/10.21929/abavet2023.15
e2022-64
Citas
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