Ribotyping of mesophilic pathogenic bacteria isolated from cuyinaze compost
Keywords:
guinea pig, Cavia porcellus, isolable microbiome, enterobacteriaAbstract
The management of excretes in guinea pig production units due to its environmental impact is a necessity, and the composting of guinea pig is a practice that is increasing due to its usefulness in the agricultural sector. The manure compost has a load of intestinal microorganisms and other environmental organisms, so knowing the diversity of bacteria is key for decision-making; e.g., to identify beneficial microorganisms in the agribusiness and those that cause diseases. The objective of the present study was to carry out a general quantitative and qualitative bacteriological analysis from guinea pig compost. The analysis was carried out by counting total Enterobacteriaceae and mesophiles, the isolates in pure culture were identified by PCR-sequencing of the variable region 3 of the 16S rRNA gene, the sequences were edited and identified by searching highly similar with 100 % identity. Phylogenetic analysis showed the presence of ten clones in two groups, the most abundant of Gram- bacteria formed by two clones and the most diverse but less abundant group of Gram+ bacteria. The guinea pig composting process allows the development of environmental mesophilic bacteria with potential impact on public health and animal health, but there are also bacteria with potential biotechnological use.
http://dx.doi.org/10.37114/abaagrof/2025.7
e2025-16
References
ABDEL-MOHSEIN HS, Sasaki T, Tada C. Nakai Y. 2011. Characterization and partial purification of a bacteriocin-like substance produced by thermophilic Bacillus licheniformis H1 isolated from cow manure compost. Anim Sci J. 82:340-51.
https://doi.org/10.1111/j.1740-0929.2010.00835.x
AGUIRRE-López EW. 2017. Producción de biofertilizante mediante fermentación de la cuyinaza por bacterias del género Lactobacillus aisladas del fermento de la chicha de la cebada. Tesis de grado. Universidad Católica Sedes Sapientiae. Perú.
https://alicia.concytec.gob.pe/vufind/Record/UCSS_fb4baaeed26d56c28fa8db85cf0d4fbf
ANDO S, Goto M, Meunchang S, Thongra-AR P, Fujiwara T, Hayashi H, Yoneyama T. 2006. Detection of nifH sequences in sugarcane (Saccharum officinarum L.) and pineapple (Ananas comosus [L.] Merr.). Soil Science & Plant Nutrition. 51:303-308.
https://doi.org/10.1111/j.1747-0765.2005.tb00034.x
ARAGADVAY-Yungán RG. 2013. Efecto de la aplicación de diferentes niveles de bacterias Rhizobium meliloti con la adición de estiercol de cuy en la producción forrajera del Medicago Sativa (Alfalfa). Tesis de grado. Escuela Superior Politécnica de Chimborazo. Ecuador. https://dspace.espoch.edu.ec/items/0ea2bba1-22bf-47c6-b1c8-40f03c1051fb
ARAVIND R, Kumar A, Eapen SJ, Ramana KV. 2009. Endophytic bacterial flora in root and stem tissues of black pepper (Piper nigrum L.) genotype: isolation, identification and evaluation against Phytophthora capsici. Lett Appl Microbiol. 48:58-64. https://doi.org/10.1111/j.1472-765x.2008.02486.x
ASH C, Farrow J, Wallbanks S, Collins M. 1991. Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small‐subunit‐ribosomal RNA sequences. Lett Appl Microbiol. 13:202-206. https://doi.org/10.1111/j.1472-765X.1991.tb00608.x
BAUTISTA-TRUJILLO GU, Solorio-Rivera JL, Renteria-Solorzano I, Carranza-German SI, Bustos-Martinez JA, Arteaga-Garibay RI, Baizabal-Aguirre VM, Cajero-Juarez M, Bravo-Patino A, Valdez-Alarcon JJ. 2013. Performance of culture media for the isolation and identification of Staphylococcus aureus from bovine mastitis. J Med Microbiol. 62:369-376. https://doi.org/10.1099/jmm.0.046284-0
DUNLAP CA. 2019. Taxonomy of registered Bacillus spp. strains used as plant pathogen antagonists. Biological Control. 134:82-86.
https://doi.org/10.1016/j.biocontrol.2019.04.011
ELBANNA K, Elnaggar S, Bakeer A. 2014. Characterization of Bacillus altitudinis as a new causative agent of bacterial Soft Rot. Journal of Phytopathology. 162:712-722. https://doi.org/10.1111/jph.12250
EPPINGER M, Bunk B, Johns MA, Edirisinghe JN, Kutumbaka KK, Koenig SSK, Creasy HH, Rosovitz MJ, Riley DR, Daugherty S, Martin M, Elbourne LDH, Paulsen I, Biedendieck R, Braun C, Grayburn S, Dhingra S, Lukyanchuk V, Ball B, UL-Qamar R, Seibel J, Bremer E, Jahn D, Ravel J, Vary PS. 2011. Genome sequences of the biotechnologically important Bacillus megaterium strains QM B1551 and DSM319. Journal of Bacteriology. 193:4199-4213. https://doi.org/10.1128/jb.00449-11
GAUTAM A. 2022. DNA and RNA Isolation Techniques for Non-Experts. Springer International Publishing. Switzerland. ISBN: 9783030942298.
GILL C, Van de Wijgert JH, Blow F, Darby AC. 2016. Evaluation of lysis methods for the extraction of bacterial DNA for analysis of the vaginal microbiota. PloS One. 11(9), e0163148. https://doi.org/10.1371/journal.pone.0163148
GREEN Michael R, Sambrook Joseph. 2001. Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Cold Spring Harbor, New York. USA. ISBN: 9781936113415. https://catalog.nlm.nih.gov/discovery/fulldisplay/alma9915882953406676/01NLM_INST:01NLM_INST
HALDER U, Banerjee A, Chaudhry V, Varshney R, Mantri S, Bandopadhyay R. 2017. Draft genome report of Bacillus altitudinis SORB11, isolated from the Indian sector of the Southern Ocean. Genome Announc. 5(23): e00339-17.
https://doi.org/10.1128/genomea.00339-17
HILDEBRAND F, Ebersbach T, Nielsen HB, Li X, Sonne SB, Bertalan M, Dimitrov P, Madsen L, Qin J, Wang J, Raes J, Kristiansen K, Licht TR. 2012. A comparative analysis of the intestinal metagenomes present in guinea pigs (Cavia porcellus) and humans (Homo sapiens). BMC Genomics. 13:514. https://doi.org/10.1186/1471-2164-13-514
INIGUEZ AL, Dong Y, Triplett EW. 2004. Nitrogen fixation in wheat provided by Klebsiella pneumoniae 342. Mol Plant Microbe Interact. 17(10):1078-1085.
https://doi.org/10.1094/mpmi.2004.17.10.1078
JACOBSEN BJ, Zidack NK, Larson BJ. 2004. The role of bacillus-based biological control agents in integrated pest management systems: plant diseases. Phytopathology. 94:1272-1275. https://doi.org/10.1094/phyto.2004.94.11.1272
KAKIMOTO T, Osawa T, Funamizu N. 2007. Antibiotic effect of amoxicillin on the feces composting process and reactivation of bacteria by intermittent feeding of feces. Bioresour Technol. 98(18):3555-3560. https://doi.org/10.1016/j.biortech.2006.11.029
KUMAR S, Stecher G, Tamura K. 2016. MEGA7: mMolecular evolutionary genetics analysis version 7.0 for Bigger Datasets. Mol Biol Evol. 33(7):1870-1874.
https://doi.org/10.1093/molbev/msw054
LEE DH, Zo YG, Kim SJ. 1996. Nonradioactive method to study genetic profiles of natural bacterial communities by PCR-single-strand-conformation polymorphism. Applied and Environmental Microbiology. 62(9):3112-3120. https://doi.org/10.1128/aem.62.9.3112-3120.1996
LINDBÄCK T, Granum PE. 2019. Bacillus cereus. In: Michael P. Doyle, Francisco Diez-Gonzalez, Colin Hill. (ed.) Food Microbiology: Fundamentals and Frontiers. 5th Edition. ISBN: 9781555819965. https://doi.org/10.1128/9781555819972.ch20
LUQUE J, Herráez A. 2001. Biología molecular e ingeniería genética: Conceptos, técnicas y aplicaciones en ciencias de la salud. Galería de imágenes.
https://catedrabiologiamolecularusal.wordpress.com/wp-content/uploads/2020/03/biologia-molecular.pdf
MEZZATESTA ML, Gona F, Stefani S. 2012. Enterobacter cloacae complex: clinical impact and emerging antibiotic resistance. Future microbiology. 7(7):887-902.
https://doi.org/10.2217/fmb.12.61
NOGI Y, Takami H, Horikoshi K. 2005. Characterization of alkaliphilic Bacillus strains used in industry: proposal of five novel species. International Journal of Systematic and Evolutionary Microbiology. 55(6):2309-2315. https://doi.org/10.1099/ijs.0.63649-0
OLIVEIRA M, VI I, Usall J, Anguera M, Abadias M. 2012. Presence and survival of Escherichia coli O157: H7 on lettuce leaves and in soil treated with contaminated compost and irrigation water. Int J Food Microbiol. 156(2):133-140.
https://doi.org/10.1016/j.ijfoodmicro.2012.03.014
PAILAN S, Gupta D, Apte S, Krishnamurthi S, Saha P. 2015. Degradation of organophosphate insecticide by a novel Bacillus aryabhattai strain SanPS1, isolated from soil of agricultural field in Burdwan, West Bengal, India. International Biodeterioration &
Biodegradation. 103:191-195. https://doi.org/10.1016/j.ibiod.2015.05.006
PATEL S, Gupta RS. 2020. A phylogenomic and comparative genomic framework for resolving the polyphyly of the genus Bacillus: Proposal for six new genera of Bacillus species, Peribacillus gen. nov., Cytobacillus gen. nov., Mesobacillus gen. nov., Neobacillus gen. nov., Metabacillus gen. nov. and Alkalihalobacillus gen. nov. International Journal of Systematic and Evolutionary Microbiology. 70(1):406-438. https://doi.org/10.1099/ijsem.0.003775
PODSCHUN R, Pietsch S, Holler C, Ullmann U. 2001. Incidence of Klebsiella species in surface waters and their expression of virulence factors. Appl Environ Microbiol. 67(7):3325-3327. https://doi.org/10.1128/aem.67.7.3325-3327.2001
RIBER L, Poulsen PH, Al-Soud WA, Skov Hansen LB, Bergmark L, Brejnrod A, Norman A, Hansen LH, Magid J, Sorensen SJ. 2014. Exploring the immediate and long-term impact on bacterial communities in soil amended with animal and urban organic waste fertilizers using pyrosequencing and screening for horizontal transfer of antibiotic resistance. FEMS Microbiol Ecol. 90(1):206-224. https://doi.org/10.1111/1574-6941.12403
ROY Chowdhury P, Mckinnon J, Wyrsch E, Hammond JM, Charles IG, Djordjevic SP. 2014. Genomic interplay in bacterial communities: implications for growth promoting practices in animal husbandry. Front Microbiol. 5:e00394.
https://doi.org/10.3389/fmicb.2014.00394
SALGADO-MORENO S, Hernández-Moreno S, Martínez-Orozco S, Chamé-Vázquez E, Borrayo-González J, Ibarra-Gudiño C, Martínez-González S. 2022. Identificación del escarabajo Alphitobius diaperinus en la cama de explotaciones de Cuyes (Cavia porcellus). Abanico Agroforestal. 4:e2022-1. http://dx.doi.org/10.37114/abaagrof/2022.6
SÁNCHEZ-Macías D, Barba-Maggi L, Morales-Delanuez A, Palmay-Paredes J. 2018. Guinea pig for meat production: A systematic review of factors affecting the production, carcass and meat quality. Meat Sci. 143:165-176.
https://doi.org/10.1016/j.meatsci.2018.05.004
SAS PE. 2000. The SAS system. SAS/STAT User’s guide version 8, 3884. ISBN: 1-58025-494-2. https://www.sfu.ca/sasdoc/sashtml/hrddoc/indfiles/57388.htm
SHAFI S, Kamili AN, Shah MA, Bandh SA, Dar R. 2017. Dynamics of bacterial class Bacilli in the deepest valley lake of Kashmir-the Manasbal Lake. Microbial pathogenesis. 104:78-83. https://doi.org/10.1016/j.micpath.2017.01.018
SHIVAJI S, Chaturvedi P, Suresh K, Reddy GSN, Dutt CBS, Wainwright M, Narlikar JV, Bhargava PM. 2006. Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. Int J Syst Evol Microbiol. 56(7):1465-1473. https://doi.org/10.1099/ijs.0.64029-0
SUNAR K, Dey P, Chakraborty U, Chakraborty B. 2015. Biocontrol efficacy and plant growth promoting activity of Bacillus altitudinis isolated from Darjeeling hills, India. Journal of basic microbiology. 55(1):91-104. https://doi.org/10.1002/jobm.201300227
TAKAHASHI T, Karita S, Yahaya MS, Goto M. 2005. Radial and axial variations of bacteria within the cecum and proximal colon of Guinea Pigs revealed by PCR–DGGE. Biosci Biotechnol Biochem. 69(9):1790-1792. https://doi.org/10.1271/bbb.69.1790
UMSAKUL K, Dissara Y, Srimuang N. 2010. Chemical, physical and microbiological changes during composting of the water hyacinth. Pakistan Journal of Biological Sciences. 13(20):985-992. https://doi.org/10.3923/pjbs.2010.985.992
VAN-THUOC D, Huu-Phong T, Thi-Binh N, Thi-Tho N, Minh-Lam D, Quillaguamán J. 2012. Polyester production by halophilic and halotolerant bacterial strains obtained from mangrove soil samples located in Northern Vietnam. Microbiology Open. 1(4):395-406. https://doi.org/10.1002/mbo3.44
WINN WC, Allen SD, Janda WM, Koneman EW, Procop GW, Schrenckenberger PC, Woods GL. 2013. Koneman. Diagnóstico microbiológico. Texto y Atlas en color, México, D.F., Editorial Medica Panamericana. México. ISBN: 978-950-06-0895-4.
https://books.google.com.mx/books?id=jyVQueKro88C&printsec=frontcover&hl=es#v=onepage&q&f=false
XICOHTENCATL-SÁNCHEZ PG, Barrera-Zúñiga S, Orozco-Orozco T, Torres-Sandoval SFM, Monsivais-Isiordia R. 2013. Parámetros productivos de cuyes (Cavia porcellus) del nacimiento al sacrificio en Nayarit, México. Abanico Veterinario. 3(1):36-43. https://dialnet.unirioja.es/servlet/articulo?codigo=7390216
YI J, Wu HY, Wu J, Deng CY, Zheng R, Chao Z. 2012. Molecular phylogenetic diversity of Bacillus community and its temporal-spatial distribution during the swine manure of composting. Appl Microbiol Biotechnol. 93:411-421. https://doi.org/10.1007/s00253-011-3425-x
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