Determinación de la calidad del semen criopreservado con lecitina de soya o yema de huevo, en machos cabríos

Silvestre Moreno-Avalos, Francisco Veliz-Deras, Guadalupe Calderon-Leyva, Viridiana Contreras-Villarreal, Juan Guillen-Muñoz, Oscar Angel-García

Resumen

El objetivo fue comparar la calidad del semen caprino criopreservado con diferentes tratamientos a base de lecitina de soya o yema de huevo. El semen fue colectado de machos cabríos Alpinos (n=4), se utilizaron dos diluyentes comerciales: AndroMed® (1% de lecitina de soya, LS); Optidyl® con 20% (v/v) de Tris-yema de huevo; TY), y un tercer diluyente a base de citrato-yema de huevo (CY), en semen fresco (SF) y después fue enfriado de 37 a 4 °C durante 2 h; semen refrigerado (SR), posteriormente se llenaron pajillas con semen y se congelaron en nitrógeno líquido a -196 °C (SC). No existieron diferencias (p>0.05) entre diluyentes en el SF respecto a motilidad masal (MM; 4.7±0.26), viabilidad espermática (VE; 74.1±1.66) y motilidad individual (MI; 62.3±4.0). En el mismo sentido, para el SR no existió diferencia (p>0.05) entre diluyentes respecto a MM=3.83±0.4, y MI= 52.1±6.0, sin embargo, la VE varió (p<0.05) de acuerdo al diluyente, observando la menor viabilidad en LS vs. CY y TY (51.0±13.0 vs 71.3±3.0 y 69.0±3.1). Respecto al SC, la MM, MI y VE favorecieron (p<0.05) al diluyente TY vs. LS y CY (2.4±0.5, 32.5±8.3, 41.3±13.0). Los resultados mostraron una mejor crio-preservación del semen caprino con el diluente Tris-yema respecto al de lecitina de soya.

Palabras clave

lecitina de soya; diluyente; semen caprino

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