Actualización de la prueba de eritrocitos micronucleados: fundamentos, significado biológico y modelos
Palavras-chave:
micronúcleos, eritrocitos, sangre periférica, genotoxicidad, citotoxicidad, inestabilidad genómicaResumo
http://dx.doi.org/10.21929/abavet2025.7
e2023-44
Referências
AGUILERA A, García-Muse T. 2013. Causes of genome instability. Annual Review of Genetics. 47:19–50. ISSN: 0066-4197. https://doi.org/10.1146/annurev-genet-111212-133232
ANGARITA MM, Torres CM, Díaz TA. 2017. Técnicas de biología molecular en el desarrollo de la investigación. Revisión de la literatura. Revista Habanera de Ciencias Médicas. 16(5):796-807. ISSN: 1729-519X.
http://scielo.sld.cu/pdf/rhcm/v16n5/en_rhcm12517.pdf
ARELLANO-GARCÍA ME, Izaguirre-Pérez ME, Molina-Noyola LD, Castañeda-Yslas IY, Luna-Vázquez-Gómez R, Torres-Bugarín O. 2020. Polydactyl hypopigmented cat with squamous cell carcinoma—A case report. Frontiers in Veterinary Science. 12(7):258. ISSN: 2297-1769. https://doi.org/10.3389/fvets.2020.00258
ARELLANO-GARCÍA ME, Torres-Bugarín O, García-García MR, García-Flores D, Toledano-Magaña Y, Sanabria-Mora CS, Castro-Gamboa S, García-Ramos JC. 2021.Genomic instability and cyto-genotoxic damage in animal species. In: updates on veterinary anatomy and physiology. Edited Catrin Sian Rutland and Samir A.A. El-Gendy. Ed. Intech Open. 1-19. ISBN: 978-1-83969-530-8.
https://www.intechopen.com/chapters/78242
BATISTA-GONZÁLEZ CM, Corona-Rivera JR, Gómez-Meda BC, Zamora-Pérez AL, Ramos-Ibarra ML, Zúñiga-González GM. 2006. Micronucleated erythrocytes in preterm newborns in relation to maternal pathology. Revista Biomédica. 17:11-16. ISSN: 2007-8447. https://www.medigraphic.com/pdfs/revbio/bio-2006/bio061c.pdf
BENVINDO-SOUZA M, Santos OE, Assis RA, Araújo SC, Borges RE, de Melo D, Souza SL. 2020. Micronucleus test in tadpole erythrocytes: Trends in studies and new paths. Chemosphere. 240:124910. ISSN: 0045-6535.
https://doi.org/10.1016/j.chemosphere.2019.124910
CALZADILLA LS, Uriarte NA, Ricardo SF, Melian SC. 2022. Consideraciones actuales sobre los teratógenos y sus efectos durante el embarazo. MEDISAN. 26(2): 381-402. ISSN: 1029-3019. http://scielo.sld.cu/pdf/san/v26n2/1029-3019-san-26-02-381.pdf
CASILLAS-FIGUEROA F, Arellano-García ME, Leyva-Aguilera C, Ruíz-Ruíz B, Luna Vázquez-Gómez R, Radilla-Chávez P, Chávez-Santoscoy RA, Pestryakov A, Toledano-Magaña Y, García-Ramos JC. 2020. Argovit™ Silver Nanoparticles Effects on Allium cepa: Plant Growth Promotion without Cyto Genotoxic Damage. Nanomaterials. 10(7):1386. ISSN: 2079-4991. https://doi.org/10.3390/nano10071386
ÇELIK, TA. 2018. Introductory Chapter: Cytotoxicity. Pp 3-6. Ed. In TechOpen. 978-1-78923-431-2. ISBN: 78-1-83881-443-4. https://www.intechopen.com/chapters/61438
CEYCA-CONTRERAS JP, Castillo-Guerrero JA, Torres-Bugarín O, García-Hernández J, Betancourt-Lozano M. 2023. Micronuclei in embryos of eight seabird species in northwestern Mexico: a biomarker of exposure to coastal pollution?. Mutation Research Genetic Toxicology and Environmental Mutagenesis. (887):503615. ISSN: 1879-3592. https://doi.org/10.1016/j.mrgentox.2023.503615
FARAG MR, Alagawany M. 2018. Erythrocytes as a biological model for screening of xenobiotics toxicity. Chemico-Biological Interactions. 5:279:73-83. ISSN: 1872-7786. https://doi.org/10.1016/j.cbi.2017.11.007
FLORES-GALVÁN MA, Daesslé LW, Arellano-García E, Torres-Bugarín O, Macías-Zamora JV, Ruíz-Campos G. 2020. Genotoxicity in fishes environmentally exposed to As, Se, Hg, Pb, Cr and toxaphene in the lower Colorado River basin, at Mexicali valley, Baja California, México. Ecotoxicology. 29(4):493-502. ISSN 1573-3017.
https://doi.org/10.1007/s10646-020-02200-9
FRANCO-RAMOS RS, López-Romero CA, Torres-Ortega H, Oseguera-Herrera D, Lamoreaux-Aguayo JP, Molina-Noyola D, Juárez-Vázquez CI, Torres-Bugarín O. 2020. Evaluation of Anti-Cytotoxic and Anti-Genotoxic effects of Nigella sativa through a micronucleus test in BALB/c mice. Nutrients. 12(5):1317. ISSN: 2072-6643.
https://doi.org/10.3390/nu12051317
GIANUZZI L. 2018. Toxicología general y aplicada. Editorial Universidad de la Plata. Pp. 5-8. ISBN: 978-950-34-1695-2. https://doi.org/10.35537/10915/71533
GÓMEZ-MEDA BC, Bañales-Martínez LR, Zamora-Perez AL, Lemus-Varela ML, Trujillo X, Sánchez-Parada MG, Torres-Mendoza BM, Armendáriz-Borunda J, Zúñiga-González GM. 2016. Micronucleated Erythrocytes in Peripheral Blood from Neonate Rats Exposed by Breastfeeding to Cyclophosphamide, Colchicine, or Cytosine-Arabinoside. BioMed Research International. 1-10. ISSN: 2314-6141. https://doi.org/10.1155/2016/9161648
GÓMEZ-MEDA, BC, Zamora-Perez, AL, Ramos-Ibarra ML, Batista-González CM, Zúñiga-González GM. 2008. Micronucleated erythrocytes in peripheral blood of newborn rabbits after exposure to cyclophosphamide during pregnancy. Scandinavian Journal of Laboratory Animal Science. 35(3):151-158. ISSN: 2002-0112.
https://doi.org/10.23675/sjlas.v35i3.146
GÓMEZ-OLIVÁN LM, Mendoza-Zenil YP, SanJuan-Reyes N, Galar-Martínez M, Ramírez-Durán N, Rodríguez Martín-Doimeadios RDC, Rodríguez-Fariñas N, Islas-Flores H, Elizalde-Velázquez A, García-Medina S, Pérez-Pastén Borja R. 2017. Geno- and cytotoxicity induced on Cyprinus carpio by aluminum, iron, mercury and mixture thereof. Ecotoxicology and Environmental Safety. 135:98-105. ISSN: 1090-2414 https://doi.org/10.1016/j.ecoenv.2016.09.037
GUIROLA FJ, Mastrapa OH, García GY, Pérez BL, Cisnero N, Batista RY. 2019. Caracterización de la intoxicación por plomo. Revisión bibliográfica. RETEL: Revista de Toxicología en Línea. 59: 39-63. ISSN: 1668-091X https://www.who.int/es/news-room/fact-sheets/detail/lead-poisoning-and-health
GUO X, Ni J, Liang Z, Xue J, Fenech MF, Wang X. 2019. The molecular origins and pathophysiological consequences of micronuclei: new insights into an age-old problem. Mutation Research/Reviews in Mutation Research. 779:1-35. ISSN: 1388-2139. https://doi.org/10.1016/j.mrrev.2018.11.001
HARABAWY SA, Mosleh YY. 2014. The role of vitamins A, C, E and selenium as antioxidants against genotoxicity and cytotoxicity of cadmium, copper, lead and zinc on erythrocytes of Nile tilapia, Oreochromis niloticus. Ecotoxicology and Environmental Safety. 104:28-35. ISSN: 1090-2414.https://doi.org/10.1016/j.ecoenv.2014.02.015
HAYASHI M. 2016. The micronucleus test—most widely used in vivo genotoxicity test. Genes and Environmental. 38, e18. ISSN: 1880-7062. https://doi.org/10.1186/s41021-016-0044-x
HEITLAND P, Köster HD. 2021. Human biomonitoring of 73 elements in blood, serum, erythrocytes and urine. Journal of Trace Elements in Medicine and Biology. 64, e126706. ISSN: 1878-3252. https://pubmed.ncbi.nlm.nih.gov/33352468/
KLAASSEN CD. 2018. Casarett & Doull’s Toxicology: The Basic Science of Poisons, 9th Edition McGraw-Hill. ISBN: 978-1-259-86374-5.
https://accesspharmacy.mhmedical.com/book.aspx?bookid=2462#194918150
LABRADA-MARTAGÓN V, Muñoz Teneriá FA, Tania Zenteno-Savín T. 2019. Standardized micronucleus assay for peripheral blood from sea turtles. Chelonian Conservation and Biology. 18(2):175-186. ISSN: 1071-8443.
https://doi.org/10.2744/CCB-1373.1
LADEIRA C, Smajdova L. 2017. The use of genotoxicity biomarkers in molecular epidemiology: Applications in environmental, occupational and dietary studies. AIMS Genet. 4:166–191. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690241/
MALACARNE IT, Alpire MES, Malinverni ACM, Ribeiro DA 2022. The use of micronucleus assay in oral mucosa cells as a suitable biomarker in children exposed to environmental mutagens: theoretical concepts, guidelines and future directions. Reviews on Environmental Health. 11, e0084. ISSN: 2191-0308. https://doi.org/10.1515/reveh-2022-0084
MARURI J, Martínez-Cortés F, Odales J, Manoutcharian K. 2022. Inestabilidad genética, origen y evolución del cáncer y la inmunoterapia personalizada. Vacunas. 23(3): 222-233. ISSN: 1576-9887. https://doi.org/10.1016/j.vacun.2022.01.004
MERCHÁN MA, Caicedo MIT, Torres AKD. 2017. Molecular biology techniques for research development. A literature review. Revista Habanera de Ciencias Médicas. 16(5):796-807. ISSN: 1729-519X.
https://www.medigraphic.com/pdfs/revhabciemed/hcm-2017/hcm175l.pdf
NARANJO-VÁZQUEZ E, Sánchez-Parada MG, Gómez-Meda BC, Zamora-Perez AL, Gallegos-Arreola MP, González-Santiago AE, Zúñiga-González GM. 2020. Effect of high-dose topical minoxidil on erythrocyte quality in SKH1 hairless mice. Animals. 10(4):731. ISSN:2076-2615. https://doi.org/10.3390/ani10040731
OLIVARES Y, Gaete H, Neaman A. 2015. Evaluación de la fitotoxicidad y la genotoxicidad de suelos agrícolas de zonas con actividades mineras de cobre de la cuenca del río Aconcagua (Chile central). Revista Internacional De Contaminación Ambiental. 31(3):237-243. ISSN: 0188-4999.
https://www.scielo.org.mx/pdf/rica/v31n3/v31n3a3.pdf
ORTEGA TM, Torres RJ, Ángel OJ. 2018. Fundamentos de citogenética humana y animal. Editorial Universidad Nacional Abierta y a Distancia UNAD. 24, 61-65. ISBN:978-958-651-651-8. https://doi.org/10.22490/9789586516518
PEDRAZZINI E, Stella F, Slavutsky I. 2022. Inestabilidad genómica en mieloma múltiple. Hematología. 25(3):1-12. ISSN: 2250-8309.
https://libros.unad.edu.co/index.php/selloeditorial/catalog/book/100
PEREDA-SOLIS M, Guillén-González C, Ramírez- Carreño K, Martínez-Guerrero J, Sierra-Franco D, Salazar-Borunda M, Torres-Bugarín O. 2022. Leukocyte profile, micronuclei, and erythrocitary nuclear protrusions in sparrows (Centronyx bairdii and Ammodramus savannarum) of the chihuahuan desert during the winter. Agrociencia. 56:46-60. ISSN: 2521-9766.
https://www.agrociencia-colpos.org/index.php/agrociencia/article/view/2710/2119
PINEDA EM. 2021. Análisis de las causas de fragmentación de ADN en células procariontes y eucariontes, Tesis de Licenciatura de la Universidad Autónoma Metropolitana Unidad Xochimilco.14-19.
https://repositorio.xoc.uam.mx/jspui/handle/123456789/26297
PUERTA-ORTIZ A, Morales-Aramburo J, Efectos biológicos de las radiaciones ionizantes- 2020. Revista Colombiana de Cardiología. 27(1):61-71. ISSN: 0120-5633.
https://rccardiologia.com/previos/RCC%202020%20Vol.%2027/RCC_2020_27_S1/RCC_2020_27_S1_061-071.pdf
RAMOS-IBARRA ML, Villa-Castellanos J, Barba-León J, Flores-Valdez M, Zavala-Aguirre JL, Torres Bugarín O. 2020. Estudio exploratorio de la genotoxicidad de vacunas recombinantes para tuberculosis bovina. Abanico Veterinario. 10(1):1-14. ISSN: 2448-6132- https://abanicoacademico.mx/revistasabanico/index.php/abanico-veterinario/article/view/249/409
RONCANCIO-VELANDIA T, Parra-Medina R, Mejia JC, Gevara-Pardo G. 2019. Hibridación in situ fluorescente (FISH) en el Instituto Nacional de Cancerología (INC) de Colombia. Experiencia de 5 años. Revista Colombiana de Cancerología. 23(1):3-11. ISSN: 0123-9015. https://doi.org/10.35509/01239015.73
RUIZ-RUIZ B, Arellano-García ME, Radilla-Chávez P, Salas-Vargas DS, Toledano-Magaña Y, Casillas-Figueroa F, Luna Vázquez-Gómez R, Pestryakov A, García-Ramos JC, Bogdanchikova N. 2020. Cytokinesis-block micronucleus assay using human lymphocytes as a sensitive tool for cytotoxicity/genotoxicity evaluation of AgNPs. ACS Omega. 5(21):12005-12015. ISSN: 2079-4991.
https://doi.org/10.1021/acsomega.0c00149
RUSSO A, Degrassi F. 2018. Molecular cytogenetics of the micronucleus: Still surprising. Mutation Research. 836 (PtA):36-40. ISSN: 1879-3592.
https://doi.org/10.1016/j.mrgentox.2018.05.011
SANDOVAL-HERRERA N, Paz J, Herrera M, Welch K. 2020. Micronucleus test reveals genotoxic effects in bats associated with agricultural activity. Environmental Toxicology and Chemistry. 40:202–207. ISSN: 1552-8618. https://doi.org/10.1002/etc.4907
SCHMID W. 1975. The micronucleus test. Mutation Research. 31(1):9-15. ISSN: 1879-3592. https://doi.org/10.1016/0165-1161(75)90058-8
SOMMER S, Buraczewska I, Kruszewski M. 2020. Micronucleus assay: The state of art, and future directions. International Journal of Molecular Sciences. 24;21(4):1534. ISSN: 1422-0067. https://www.mdpi.com/1422-0067/21/4/1534
STEVENSON A, Lopez D, Khoo P, Kalaria RN, Mukaetova EB. 2017. Exploring erythrocytes as blood biomarkers for Alzheimer's disease. Journal of Alzheimer's Disease. 60(3):845-857. ISSN: 1875-8908. https://pubmed.ncbi.nlm.nih.gov/28984593/
TERRADAS M, Martín, M, Genescà A. 2016. Impaired nuclear functions in micronuclei results in genome instability and chromothripsis. Archives of Toxicology. 90(11):2657–2667. ISSN: 1432-0738. https://doi.org/10.1007/s00204-016-1818-4
STOPPER H, Bankoglu EE, Marcos R, Pastor S. 2020. Micronucleus frequency in chronic kidney disease patients: A review. Mutation Research - Reviews in Mutation Research. 786, e108340. ISSN:1388-2139.
https://doi.org/10.1016/j.mrrev.2020.108340
TORRES-BUGARÍN O, Arias-Ruíz L. 2023. Micronúcleos: Actualización del papel en la inestabilidad genética, inflamación, envejecimiento y cáncer. Revisión panorámica. Revista Biomédica. 34(2):208-223. ISSN: 20078447.
https://doi.org/10.32776/revbiomed.v34i2.1101
TORRES-BUGARÍN O, Carillo-GC, Armijo GJ. 2019. Evaluación de genotóxicos ambientales mediante la prueba de micronúcleos en sangre periférica. En Ecología y Salud de la fauna silvestre Avances de investigación. Colección Investigadores. UJED. Ed. 2. ISBN: 978-607-503-223-8.
http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/589
TORRES-BUGARIN O, Ramos-Ibarra M, Flores-G A, Ruíz BS, Zavala-Cerna MG. 2015. La prueba de micronúcleos: Biomarcador de contaminación genotóxica, mutagénica y/o teratogénica. En Pacífico Mexicano, Contaminación e Impactos Ambientales: Diagnóstico y Tendencias. 2015.819-8-48. ISBN: 978-607-7887-94-2.
https://cibnor.repositorioinstitucional.mx/jspui/handle/1001/589
TORRES-BUGARÍN O, Zavala-Cerna M, Nava-Zavala A, Flores-García A, Ramos-Ibarra M. 2014. Potential uses, limitations, and basic procedures of micronuclei and nuclear abnormalities in buccal cells. Disease Markers. 2014, e956835. ISSN: 1875-8630. https://doi.org/10.1155/2014/956835
VALBUENA D, Meléndez FM, Villegas V, Sánchez M, Rodón LM. 2020. Daño celular y genético como determinantes de la toxicidad de los plaguicidas. Ciencia en Desarrollo. 11(2):25-42. ISSN: 2462-7658. https://doi.org/10.19053/01217488.v11.n2.2020.11245
VALENZUELA-SALAS LM, Girón-Vázquez NG, García-Ramos JC, Torres-Bugarín O, Gómez C, Pestryakov A, Villarreal-Gómez LJ, Toledano-Magaña Y, Bogdanchikova N. 2019. Antiproliferative and antitumor effect of non-genotoxic silver nanoparticles on melanoma models. Oxidative Medicine and Cellular Longevity. 1-12. ISSN: 1942-0994. https://doi.org/10.1155/2019/4528241
YAMUNAQUE CL, García BF, Serquén-LL. 2019. Ensayo cometa. Revista Experiencia en Medicina del Hospital Regional Lambayeque. 5(3)162-163. ISSN: 2412-4214. https://rem.hrlamb.gob.pe/index.php/REM/article/view/374/222
ZAMORA-PEREZ A, Camacho-Magaña C, Gómez-Meda B, Ramos-Ibarra M, Batista-González C, Zúñiga-González G. 2006. Importance of spontaneous micronucleated erythrocytes in bottlenose dolphin (Tursiops truncatus) to marine toxicology studies. Acta Biologica Hungarica. 57(4):441-448. ISSN:0236-5383.
https://pubmed.ncbi.nlm.nih.gov/17278706/
ZAMORA-PEREZ AL, Luna-Aguirre J, Zúñiga-González GM, Torres-Bugarín O, Torres-Mendoza BM, Gallegos-Arreola MP, Ortiz-García RG, Gutiérrez-Sevilla JE, Gómez-Meda BC. 2021. Micronuclei and nuclear buds induced by cyclophosphamide in Crocodylus moreletii as useful biomarkers in aquatic environments. Animals. 11(11): 3178. ISSN: 2076-2615. https://doi.org/10.3390/ani11113178
ZÚÑIGA G, Torres-Bugarín O, Ramírez-Muñoz MP, Delgado-Lamas JL, De Loza-Saldaña R, Cantú JM. 1996. Micronucleated erythrocytes in splenectomized with and without chemotherapy. Mutation Research. 12;361(2-3):107-112. ISSN: 1879-3592. https://doi.org/10.1016/s0165-1161(96)90244-7
ZÚÑIGA-GONZÁLEZ GM, Gómez-Meda BC, Zamora-Perez AL, Martínez-González MA, Muñoz de Haro IA, Pérez-Navarro AE, Armendáriz-Borunda J, Gallegos-Arreola MP. 2015. Micronucleated erythrocytes in newborns of rat dams exposed to ultraviolet-A light during pregnancy, protection by ascorbic acid supplementation. Mutation Research/Genetic Toxicology. 782:36-41. ISSN: 1383-5718.
https://doi.org/10.1016/j.mrgentox.2015.03.013
ZÚÑIGA-GONZÁLEZ GM, Torres-Bugarín O, Zamora-Perez AL, Gómez-Meda BC, Ramos-Ibarra ML, Gallegos-Arreola P, Flores-García A, López-Uribe A. 2003. Induction of micronucleated erythrocytes in mouse peripheral blood after cutaneous application of 5-fluorouracil. Archives of Medical Research. 34:141-144. ISSN: 1873-5487. https://doi.org/10.1016/S0188-4409(02)00470-8
ZÚÑIGA-GONZÁLEZ GM, Gómez-Meda BC, Zamora-Perez AL, Ramos-Ibarra ML, Batista-González CM, Lemus-Varela ML, Rodríguez-Avila JL, Gallegos-Arreola MP. 2005. Micronucleated erythrocyte frequencies in old and new world primates: measurement of micronucleated erythrocyte frequencies in peripheral blood of Callithrix jacchus as a model for evaluating genotoxicity in primates. Environmental and Molecular Mutagenesis. 46(4):253-9. ISSN:1098-2280. https://doi.org/10.1002/em.20154
