Determination of non-esterified fatty acids, β-hydroxybutyrate, triacylglycerol and cholesterol during negative energy balance in Holstein cows
Keywords:
dairy cowAbstract
The objective of the present work was to quantify the concentration of non-esterified fatty acids, β-
hydroxybutyrate, triacylglycerol and cholesterol during the negative energy balance, in Holstein cows. The
study was carried out by sampling and analyzing 250 blood sera obtained from 50 Holstein cows. After
delivery, the first sampling was performed at 7 d, the second at 15 d and the three remaining at 30, 45 and
60 d. Mean values for non-esterified fatty acids, β-hydroxybutyrate, triacylglycerol and cholesterol were
determined. The resulting data set was analyzed by Gaussian distribution and descriptive statistics.
Confidence intervals of 95% were established. The comparison between groups was carried out by Analysis
of Variance and frequency histogram. The non-esterified fatty acids, β-hydroxybutyrate and triacylglycerol, are consistent with the values reported internationally. The cholesterol concentration showed a slightly
higher value than the references. The AGNE did not present differences between groups, the β-
hydroxybutyrate registered its highest value at 15 d postpartum, and lowest at 60 d postpartum.
Triacylglycerol and cholesterol showed their highest concentration at 60 d postpartum, and the lowest at 15
d postpartum. The calculated confidence intervals could be used at the herd level to detect alert situations
when at least 5% of the cows in the sample fall outside the reference range.
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