Evaluación de la adición de quercetina y vitamina E al medio de criopreservación de semen ovino sobre la fertilidad in vivo

Eduardo Jiménez-Aguilar

Authors

Eduardo Jiménez-Aguilar Universidad Autónoma de Ciudad Juárez https://orcid.org/0000-0003-0847-529X

Keywords:

antioxidant, freezing, gestation rate

Abstract

Cryopreservation induces oxidative stress that has adverse effects on post-thawed semen quality. The objective was to evaluate the effect of adding quercetin to sheep semen cryopreservation medium. Semen was collected from three sheep stallions by artificial vagina, and a commercial diluent was used. Treatments were: control; quercetin 200 µM; vitamin E 100 µM; and the quercetin and vitamin E combination. Vitality, motility, acrosome integrity and fertility were evaluated in vivo. Numerical variables were analyzed with ANOVA and gestation rate with chi-square. In the evaluation of sperm motility characteristics, no significant difference was found between treatments. The treatment with the highest percentage of live spermatozoa with intact acrosome was quercetin 200 µM (22.33±2.51%) compared to the other treatments (P<0.05). In vivo fertility was not statistically significant, but a numerical difference in the percentage of gestation was found with the addition of 200 µM quercetin (51.92%) compared to the other treatments. In conclusion, the addition of 200 µM quercetin to the ovine semen cryopreservation medium improved the vitality and integrity of the acrosome, but not the in vivo fertility percentage.

http://dx.doi.org/10.21929/abavet2021.36

e2021-38.

References

ABDI-BENEMAR H, Jafaroghli M, Khalili B, Zamiri MJ, Ezazi H, Shadparvar AA. 2015. Effects of DHA supplementation of the extender containing egg yolk and α- tocopherol on the freezability and post-thawing fertility of ram semen. Small Ruminant Research. 130:166–170. ISSN: 0921-4488. https://doi.org/10.1016/j.smallrumres.2015.06.013

AHMED H, Jahan S, Salman MM, Ullah F. 2019. Stimulating effects of Quercetin (QUE) in tris citric acid extender on post thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa. Theriogenology. 134:18-23. ISSN: 0093-691X. https://doi.org/10.1016/j.theriogenology.2019.05.012

AITKEN RJ. 2017. Reactive oxygen species as mediators of sperm capacitation and pathological damage. Molecular Reproduction and Development. 84:1039-1052. ISSN: 1098-2795. https://doi.org/10.1002/mrd.22871

ALLAI L, Anass B, Da silva M, Nasser B, El-Amari B. 2018. Supplementation of ram semen extender to improve seminal quality and fertility rate. Animal Reproduction Science. 192:6-17. ISSN: 0378-4320. https://doi.org/10.1016/j.anireprosci.2018.03.019

ALHAMADA M, Debus N, Lurette A, Bocquier F. 2017. Automatic oestrus detection system enables monitoring of sexual behaviour in sheep. Small Ruminant Research. 149:105–111. ISSN: 0921-4488. https://doi.org/10.1016/j.smallrumres.2017.02.003

ARDESHIRNIA R, Zandi M, Sanjabi MR. 2017. The effect of quercetin on fertility of frozen-thawed ram epididymal spermatozoa. South African Journal of Animal Science. 47:237-244. ISSN: 2221-4062. http://dx.doi.org/10.4314/sajas.v47i2.16

BANDAY MN, Lone FA, Rasool F, Rashid M, Shiraki A. 2017. Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation. Cryobiology. 74:25-30. ISSN: 0011-2240. https://doi.org/10.1016/j.cryobiol.2016.12.008

BENHENIA K, Lamara A, Fatmi S, Iguer-Ouada M. 2016. Effect of cyclodextrins, cholesterol and vitamin E and their complexation on cryopreserved epididymal ram semen. Small Ruminant Research. 141:29–35. ISSN: 0921-4488. https://doi.org/10.1016/j.smallrumres.2016.06.009

BERKOVICH L, Earon G, Ron I, Rimmon A, Vexler A, Lev-Ari S. 2013. Moringa oleifera aqueous leaf extract down-regulates nuclear factor- kappa B and increases citotoxic effect of chemotherapy in pancreatic cancer cells. BMC Complementary and Alternative Medicine. 13:212-218. ISSN: 2662-7671. https://doi.org/10.1186/1472-6882-13-212

CÂMARA DR, Silva SV, Almeida FC, Nunes JF, Guerra MMP. 2011. Effects of antioxidants and duration of pre-freezing equilibration on frozen-thawed ram semen. Theriogenology. 76:342–350. ISSN: 0093-691X.

https://doi.org/10.1016/j.theriogenology.2011.02.013

EL-KHAWAGAH ARM, Kandiel MMM, Samir H. 2020. Effect of quercetin supplementation in extender on sperm kinematics, extracellular enzymes release, and oxidative stress of egyptian buffalo bulls frozen–thawed semen. Frontiers in Veterinary Science. 7:604460. ISSN: 2297-1769. https://doi.org/10.3389/fvets.2020.604460

GIBB Z, Butler T, Morris L, Maxwell W, Grupen C. 2013. Quercetin improves the post thaw characteristics of cryopreserved sex-sorted and non-sorted stallion sperm. Theriogenology. 79:1001-1009. ISSN: 0093-691X.

https://doi.org/10.1016/j.theriogenology.2012.06.032

KARIMFAR MH, Niazvand F, Haghani K, Ghafourian S, Shirazi R, Bakhtiyari S. 2015. The protective effects of melatonin against cryopreservation-induced oxidative stress in human sperm. International Journal of Inmunopathology and Pharmacology. 28:69-76. ISSN: 2058-7384. https://doi.org/10.1177/0394632015572080

MARTINEZ–PASTOR F, Johannisson A, Gil J, Kaabi M, Anel L, Paz P, Rodriguez–Martinez H. 2004. Use of chromatin stability assay, mitochondrial stain JC–1, and fluorometric assessment of plasma membrane to evaluate frozen–thawed ram semen. Animal Reproduction Science. 84:121-133. ISSN: 0378-4320.

https://doi.org/10.1016/j.anireprosci.2003.12.006

MASOUDI R, Shahnen AZ, Towhidi A, Kohram H, Akbarisharif A, Sharafi M. 2017. Fertility response of artificial insemination methods in sheep with fresh and frozen-thawed semen. Cryobiology. 74:77-80. ISSN: 0011-2240. https://doi.org/10.1016/j.cryobiol.2016.11.012

MATA-CAMPUZANO M, Álvarez-Rodríguez M, Álvarez M, Tamayo-Canul J, Anel L, de Paz P, Martínez-Pastor F. 2015. Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender. Theriogenology. 83:520-528. ISSN: 0093-691X.

https://doi.org/10.1016/j.theriogenology.2014.10.018

MCNIVEN M, Richardson G. 2006. Effect of quercetin on capacitation status and lipid peroxidation of stallion spermatozoa. Cell Preservation Technology. 4:169-177. ISSN: 1557-8119. https://doi.org/10.1089/cpt.2006.4.169

MOTLAGH MK, Sharafi M, Zhandi M, Mohammadi-Sangcheshmeh A, Skakeri M, Soleimani M, Zeinoaldini S. 2014. Antioxidant effect of rosemary (Rosmarinus officinalis L.) in soybean lecithin-based semen extender following freeze-thawing process of ram sperm. Cryobiology. 69:217-22. ISSN: 0011-2240.

https://doi.org/10.1016/j.cryobiol.2014.07.007

RESTREPO G, Montoya JD, Rojano B. 2016. Antioxidant capacity and post-thaw quality of stallion semen cryopreserved with quercetin and ergothioneine. Revista de la Facultad de Medicina Veterinaria y de Zootecnia. 63:167-178. ISSN 0120-2952. https://doi.org/10.15446/rfmvz.v63n3.62747

SARLÓS P, Molnár A, Kókai M, Gábor GY, Rátky J. 2002. Comparative evaluation of the effect of antioxidants in the conservation of ram semen. Acta Veterinaria Hungarica. 50:235–245. ISSN: 1588-2705. https://doi.org/10.1556/avet.50.2002.2.13

SEIFI-JAMADI A, Kohram H, Shahneh AZ, Ansari M, Macías-García B. 2016. Quercetin ameliorate motility in frozen-thawed turkmen stallions sperm. Journal of Equine Veterinary Science. 45:73–77. ISSN: 0737-0806. https://doi.org/10.1016/j.jevs.2016.06.078

SEIFI-JAMADI A, Ahmad E, Ansari M, Kohram H. 2017. Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen. Cryobiology. 75:15-20. ISSN: 0011-2240. https://doi.org/10.1016/j.cryobiol.2017.03.002

SILVA E, Cajueiro J, Silva S, Soares P, Guerra M. 2012. Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm. Theriogenology. 77:1722-1726. ISSN: 0093-691X. https://doi.org/10.1016/j.theriogenology.2011.11.023

SOUSA PC, Santos EAA, Silva AM, Bezerra JAB, Souza ALP, Lima GL, Oliveira MF, Silva AR. 2016. Identification of ultrastructural and functional damages in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation. Pesquisa Veterinária Brasileira. 36:767-774. ISSN: 1678-5150. https://doi.org/10.1590/S0100-736X2016000800015

STOJANOVIĆ S, Sprinz H, Brede O. 2001. Efficiency and mechanism of the antioxidant action of trans-resveratrol and its analogues in the radical liposome oxidation. Archives of Biochemistry and Biophysics. 391:79–89. ISSN: 0003-9861.

https://doi.org/10.1006/abbi.2001.2388