Molecular and serological characterization of Anaplasma marginale in the northeast transboundary region of Mexico

Authors

Keywords:

bovine, infection, anaplasmosis, diagnostic, PCR

Abstract

Anaplasmosis is a disease caused by rickettsiae Anaplasma spp, however Anaplasma marginale is the causal agent of bovine anaplasmosis whose pathogenicity is the highest among all species.  Nevertheless, in the northeastern region of Mexico there is no updated information that allows characterize the epidemiology of the disease, therefore in the present study 50 bovines coming from the transboundary region Mexico-USA in the state of Tamaulipas were tested during the period from April to October of the year 2021. The diagnostic was carried out by blood smears, PCR and ELISA and the results obtained allow corroborate the presence of A. marginale in some sampled municipalities, in addition to this we confirm that PCR continues to be the most efficient method for the diagnostic of this pathogen due to the high sensitivity, even before the animals show the clinical signs of the disease or that the blood parameters present some variation as result of the infection.

http://dx.doi.org/10.21929/abavet2024.15      

e2022-71

https://www.youtube.com/watch?v=ZPKXVtgYmp0

References

BERISTAIN-RUIZ D, Vital-García C, Figueroa-Millán JV, Lira-Amaya JJ, Garza-Hernández JA, Sánchez-Ayala JR, Flores-Ceballos S, Rodríguez-Alarcón CA, Olivas-Sánchez MP, Pons-Monarrez G. 2021. Molecular Detection of Tick-Borne Pathogens in American Bison (Bison bison) at El Uno Ecological Reserve, Janos, Chihuahua, Mexico. Pathogens. 10(11):1-16. https://doi.org/10.3390/pathogens10111428

BISEN S, Aftab A, Jeeva K, Silamparasan M, Yadav S, Chandra D, Sankar M, Garg R, Raina OK. 2021. Molecular and serological detection of Anaplasma infection in carrier cattle in north India. Veterinary parasitology. 24:1-7.

https://doi.org/10.1016/j.vprsr.2021.100550

BRAYTON KA. 2012. Transmisión de Anaplasma marginale por garrapatas. Revista Mexicana de Ciencias Pecuarias. 3(1):41-50.

https://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-11242012000500006

CABEZAS-CRUZ A, de la Fuente J. 2015. Anaplasma marginale major surface protein 1a: a marker of strain diversity with implications for control of bovine anaplasmosis. Ticks and tick-borne diseases. 6(3):205-210. https://doi.org/10.1016/j.ttbdis.2015.03.007

CASTAÑEDA-ORTIZ EJ, Ueti MW, Camacho-Nuez M, Mosqueda JJ, Mousel MR, Johnson WC, Palmer GH. 2015. Association of Anaplasma marginale strain superinfection with infection prevalence within tropical regions. PLoS One. 20(10):1-8. https://doi.org/10.1371/journal.pone.0129415

CORONA B, Martinez S. 2011. Deteccción de Anaplasma marginale en bovinos, mediante la amplificación por PCR del gen MSP5. Revista de Salud Animal. 33(1):24-31. http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S0253-570X2011000100004

CURTIS AK. Kleinhenz MD, Anantatat T, Martin MS, Magnin GC, Coetzee JF, Reif KE. 2021. Failure to eliminate persistent Anaplasma marginale infection from cattle using labeled doses of chlortetracycline and oxytetracycline antimicrobials. Veterinary Sciences. 8(11):1-13. https://doi.org/10.3390/vetsci8110283

DANTAN-GONZALEZ E, Quiroz-Castañeda RE, Aguilar-Díaz H, Amaro-Estrada I, Martínez-Ocampo F, Rodríguez-Camarillo S. 2022. Mexican Strains of Anaplasma marginale: a first comparative genomics and phylogeographic analysis. Pathogens. 11(8):1-15. https://doi.org/10.3390/pathogens11080873

ELEFTHERIOU A, Cole D, Kieffer J, Pesapane R. 2022. Molecular prevalence of Anaplasma marginale and associated risk factors in beef cattle herds from Ohio: a cross-sectional study. Journal of the American Veterinary Medical Association. 1(56). https://doi.org/10.2460/javma.22.05.0204

FERREIRA G, Canozzi M, Peripolli V, Moura G, Sanchez J, Martins C. 2022. Prevalence of bovine Babesia spp., Anaplasma marginale, and their co-infections in Latin America: Systematic review-meta-analysis. Ticks and tick-borne diseases. 13(4).

https://doi.org/10.1016/j.ttbdis.2022.101967

GHOSH P, Saleh M, Sundstrom K, Lentile M, Little S. 2021. Ixodes spp. from Dogs and Cats in the United States: Diversity, Seasonality, and Prevalence of Borrelia burgdorferi and Anaplasma phagocytophilum. Vector borne and zoonotic diseases. 21(1):11-19. https://doi.org/10.1089/vbz.2020.2637

GUILLEMI E. De la fourniere S, Orozco M, Peña J, Correa J, Lopez L, Paoletta M, Corona B, Pinarello V, Wilkowsky S, Farber M. 2016. Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines. Parasites & Vectors. 9(305):1-9. https://doi.org/10.1186/s13071-016-1555-9

KAMANI J, Irene S, Qasim A, Olubade T, Abasiama M, Gajibo A, Balami P, Shands M, Harrus S. 2022. Nucleotide sequence types (ntSTs) of Anaplasma marginale in cattle in Nigeria based on the major surface protein 5 (msp5) gene. Acta Tropica. 233. https://doi.org/10.1016/j.actatropica.2022.106544

LIRA-AMAYA J, Santamaria R, Castañeda R, Martínez G, Polanco D, Rojas C, Alvarez J, Figueroa J. 2022. Molecular Identification of Babesia spp. and Anaplasma marginale in Water Buffaloes in Veracruz and Tabasco, Mexico: A Retrospective Study. Microorganisms. 10(9):1-10. https://doi.org/10.3390/microorganisms10091702

NOAMAN V, Shayan P. 2010. Comparison of Microscopy and PCR-RFLP for detection of Anaplasma marginale in carrier cattle. Iranian Journal of Microbiology. 2(2):89-94. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279773/

OGATA S, Pereira J, Johnny L, Carolina H, Matsuno K, Orba Y, Sawa H, Kawamori F, Nonaka N, Nakao R. 2021. Molecular survey of Babesia and Anaplasma infection in cattle in Bolivia. Veterinary Sciences. 8(9):1-12. https://doi.org/10.3390/vetsci8090188

OKAFOR C, Collins S, Daniel J, Coetzee J, Whitlock B. 2019. Seroprevalence of bovine Anaplasmosis in Georgia. Veterinary parasitology. 15: e100258.

https://doi.org/10.1016/j.vprsr.2018.100258

OLAFSON P, Thomas D, May M, Buckmeier B, Duhaime R. 2018. Tick vector and disease pathogen surveillance of nilgai antelope (Boselaphus tragocamelus) in southeastern Texas, USA. Journal of wildlife diseases. 54(4):734-744.

https://doi.org/10.7589/2017-09-239

PRADEEP R, Nimisha M, Sruthi M, Vidya P, Amrutha B, Kurbet P, Kumar K, Varghese A, Deepa C, Dinesh C, Chandrasekhar L, Juliet S, Pradeepkumar P, Ravishankar C, Ghosh S, Ravindran R. 2019. Molecular characterization of South Indian field isolates of bovine Babesia spp. and Anaplasma spp. Parasitology Research. 118(2):617-630. https://doi.org/10.1007/s00436-018-6172-4

QUIROZ-CASTAÑEDA R, Amaro-Estrada I, Rodríguez-Camarillo S. 2016. Anaplasma marginale: diversity, virulence, and vaccine landscape through a genomics approach. BioMed Research International. 2016:1-18. https://doi.org/10.1155/2016/9032085

REZENDE-ARAUJO T, Leite Mota Júnior M, Sampaio Vilela T, Bittecourt A, Azevedo Santos H, Fampa P. 2021. First report of the presence of Anaplasma marginale in different tissues of the stable-fly Stomoxys calcitrans (Linnaeus, 1758) in Rio de Janeiro state, Brazil. Veterinary parasitology. 23: e100515.

https://doi.org/10.1016/j.vprsr.2020.100515

RODRIGUEZ-CAMARILLO S, Quiroz-Castañeda R, Aguilar-Díaz H, Vara-Pastrana J, Pescador-Pérez D, Amaro-Estrada I, Martínez-Ocampo F. 2020. Immunoinformatic Analysis to Identify Proteins to Be Used as Potential Targets to Control Bovine Anaplasmosis. International Journal of Microbiology. 27:1-8.

https://doi.org/10.1155/2020/8882031

SARLI M, Thompson C, Novoa M, Valentini B, Mastropaolo M, Echaide I, de Echaide S, Primo M. 2020. Development and evaluation of a double-antigen sandwich ELISA to identify Anaplasma marginale-infected and A. centrale-vaccinated cattle. Journal of veterinary diagnostic investigation: official publication of the American Association of Veterinary Laboratory Diagnosticians. 32(1):70-76.

https://doi.org/10.1177/1040638719892953

SILAGHI C, Santos A, Gomes J, Christova I, Matei I, Walder G, Domingos A, Bell-Sakyi L, Sprong H, von Loewenich F, Oteo J, de la Fuente J, Dumler J. 2017. Guidelines for the direct detection of Anaplasma spp. in diagnosis and epidemiological studies vector borne and zoonotic diseases. Vector borne and zoonotic diseases. 17(1):12-22. https://doi.org/10.1089/vbz.2016.1960

WATTHANADIREK A, Junsiri W, Minsakorn S, Poolsawat N, Srionrod N, Khumpim P, Chawengkirttikul R, Anuracpreeda P. 2021. Molecular and recombinant characterization of major surface protein 5 from Anaplasma marginale. Acta tropica. 220. https://doi.org/10.1016/j.actatropica.2021.105933

ZABEL T, Agusto F. 2018. Transmission dynamics of bovine anaplasmosis in a cattle herd. Interdisciplinary Perspectives on Infectious Diseases. 1:1-18.

https://doi.org/10.1155/2018/4373981

ZEB J, Shams S, Din I, Ayaz S, Khan A, Nasreen N, Khan H, Khan M, Senbill, H. 2020. Molecular epidemiology and associated risk factors of Anaplasma marginale and Theileria annulata in cattle from North-western Pakistan. Veterinary Parasitology. 279:e109044. https://doi.org/10.1016/j.vetpar.2020.109044

Published

2024-09-21

Issue

Section

Case Studies