In vitro induction of callogenesis and organogenesis in explants of Krameria erecta Willd
Keywords:
Krameria erecta, tissue culture, indolebutyric acid, explantsAbstract
The objective of the present investigation was to evaluate in vitro the germination and induction of callogenesis and organogenesis in explants of Krameria erecta. The culture medium used was WPM / 50 with half the salts and made up of vitamins, sucrose and indolebutyric acid (IAB), in concentrations (0, 0.5, 1.0, 1.5 and 2 mgL1). The explants were incubated in a controlled environment of 25 ° C and photo period of 16 light hours. The experimental design was completely randomized with a 5x3 factorial arrangement with an ANDEVA (P <0.05) and the Tukey mean test. In vitro germination did not present significant differences, showing from 79 to 83%. There were significant differences in seedling height, the concentrations of 1.5 and 2.0 mgL1 of AIB were higher with 11.60 to 11.65 cm. The height and width of callus in axillary bud and leaf presented significant differences in concentrations of 1.0 and 2 mgL1 of AIB. Regarding the number of root in the leaf it was from 0.50 to 2.50, showing significant differences in the concentrations, in the axillary bud there was no root, therefore there were no significant differences. In contamination, there were no significant differences presented below 12.50%. According to the disinfection with alcohol and sodium hypochlorite (NaClO), there was no oxidation in seeds, but in axillary bud and leaves.
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