Stimulation of callus and organogenesis in Leucaena greggii S. Watson explants

Abstract

Leucaena greggii belongs to the Fabaceae family and is a species native to northeastern Mexico. It has uses as forage, medicinal plants, timber, and food for humans. Currently, it is threatened by habitat loss and overgrazing. The objective of this research was to stimulate callus formation and organogenesis in Leucaena greggii explants (stem, axillary bud, and leaf). For this purpose, WPM culture medium supplemented with indolebutyric acid (IBA) was used at different concentrations (0, 1.25, 2.5, and 3.75 mg L⁻¹). An ANOVA and a Tukey-Kramer test for mean comparison were applied. The results showed no significant differences between treatments for callus formation induction, with average callus heights of 4.514 mm and widths of 6.042 mm. The treatment that resulted in the greatest growth in callus height and width was the axillary bud with a concentration of 2.5 mg L⁻¹. Furthermore, organogenesis was also observed in the axillary bud and stem, as seen in five explants with concentrations of 2.5 and 3.75 mg L⁻¹. The overall contamination percentage was below 2%, and the oxidation percentage was 100% in the leaf explant, which is more susceptible to stress than the other plant organs. It should be noted that the disinfection protocol for this species was appropriate.

http://dx.doi.org/10.37114/abaagrof/2026.2                                                              

 e2026-2

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