Obtaining callogenesis and organogenesis of Ibervillea sonidoe (S. Watson) Greene

Abstract

The research aimed to induce the production of callogenesis and organogenesis in vitro in Ibervillea sonorae explants. for this purpose, WPM was used as a culture medium with the addition of indole-3-butyric acid (AIB) and 6-benzylaminopurine (BAP) (0.0, 5.0, 10.0 and 15.0 mgL^-1) and a phytohormonal combination 10.0 mgL^-1 AIB with BAP (5.0, 10.0 and 15.0 mgL^-1), for the statistical analysis a randomized arrangement and a Tukey-Kramer mean test were used (p < 0.05), According to the results, these showed that there are significant differences, obtaining stem calluses with 10.00 mgL^-1 AIB and the combination 10.00 mgL^-1 of AIB/5.00 mgL^-1 BAP, presenting a callus height and width of 2.94 mm and 2.33 mm. In organogenesis with the phytohormonal combination of 10.00 mgL^-1 IBA/5.00 mgL^-1 BAP in axillary bud, a sprouting height of 3.34 and a root-stem length of 0.52 mm was obtained with 1.00 mgL^-1 IBA. Contamination was present at 24.94%, and oxidation was 4.88%. Following the above, in vitro callogenesis and organogenesis of I. sonorae contributes to the conservation of phytogenetic material, reducing extraction and implementing techniques for the propagation of the species.

http://dx.doi.org/10.37114/abaagrof/2025.5                                  

 e2025-3

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