Respuesta serológica contra Mannheimia haemolytica y su leucotoxina en conejos suplementados con selenio

Víctor Díaz-Sánchez, Lina Ciriaco-Solano, Gabriela Rodríguez-Patiño, Raquel López-Arellano, Alma Revilla-Vázquez, José Morales-Álvarez

Resumen

La selenodeficiencia tiene un impacto negativo en la respuesta inmune de los animales. El objetivo del trabajo fue evaluar la suplementación con selenio y su efecto sobre la respuesta contra Mannheimia haemolytica y su leucotoxina. Se utilizaron 21 conejos; éstos fueron distribuidos en tres grupos (n = 7); A: se les administró selenio más una bacterina-toxoide; B: se les administró bacterina-toxoide. C: considerado control. Se estimó el contenido de selenio en sangre por espectrofotometría de absorción atómica. Se evaluó la respuesta a los antígenos a través una ELISA. Se realizó un análisis de varianza y de Tukey para determinar la significancia estadística, considerando un valor P<0.05. En la cuantificación de selenio se observó una diferencia entre A con respecto a B y C (P<0.05). En la evaluación de IgG contra M. haemolytica, hubo una diferencia entre A con respecto a B y C (P<0.05). Para IgG contra la leucotoxina, no se observaron diferencias entre A y B (P>0.05), pero si de estos con respecto a C (P<0.05). En conclusión, los animales suplementados tuvieron mayores concentraciones de selenio. Ésta tuvo efectos positivos sobre la respuesta contra M. haemolytica, sin embargo, no se encontraron diferencias para la respuesta contra la leucotoxina.

Palabras clave

Mannheimia haemolytica; selenio; conejos; inmunidad

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