Efecto de los antioxidantes Trolox y Crocina sobre la criopreservación del semen de ovino Pelibuey

Itzel Rodríguez-Gutiérrez, Álvaro Domínguez-Rebolledo, Henry Loeza-Concha, Carlos Castellanos-Zacarías, Maximiliano Tun-Moo, Julio Ramón-Ugalde


El objetivo del estudio fue evaluar el efecto de los antioxidantes Trolox y Crocina en el diluyente de congelación sobre la criopreservación del semen de ovino Pelibuey. Los eyaculados obtenidos se mezclaron, dividieron y congelaron en 3 tratamientos: T1: Trolox 1mM; T2: Crocina 1mM; TT: Testigo (sin antioxidante). Se analizó a las 0 horas (descongelación) y 6 horas a 37 °C, la motilidad total (MT) y progresiva (MP), la viabilidad espermática, la actividad mitocondrial, la integridad de los acrosomas y de la membrana de la cola (Host). Los datos se analizaron con un ANOVA para un diseño completamente al azar y una prueba de Tukey para la comparación de medias. En ninguna de las dos horas se encontraron diferencias significativas en los tratamientos (P>0.05). Sin embargo, de 0 a 6h, la MT se comportó de forma similar en el T1 y T2, pero no así en el TT (P<0.05), por el contrario, la integridad de los acrosomas en los tres tratamientos fue similar (P<0.05). La MP, viabilidad, actividad mitocondrial y Host disminuyeron con el tiempo de forma similar en los tres tratamientos. Los antioxidantes Trolox y Crocina a 1mM en el diluyente de congelación, permiten mantener la MT y la integridad de los acrosomas durante 6 h de incubación a 37 °C.

Palabras clave

Radicales libres, diluyente, esperma y congelación


AGARWAL A, Saleh RA, Bedaiwy MA. 2003. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertility and Sterility. 79(4): 829-843. ISSN: 0015-0282. http:/dx.doi.org/10.1016/S0015-0282(02)04948-8

Aitken RJ. 1999. The Amoroso Lecture. The human spermatozoon-a cell in crisis?. Journal of Reproduction and Fertility. 115(1): 1-7. ISSN: 2228-5482. http:/dx.doi.org/10.1530/jrf.0.1150001

CHATTERJEE S, Gagnon C. 2001. Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Molecular Reproduction and Development. 59(4): 451-458. ISSN: 1098-2795. http:/dx.doi.org/10.1002/mrd.1052

CHEN H, Chow, PH, Cheng SK, Cheung AL, Cheng LY, WS O. 2003. Male genital tract antioxidant enzymes: Their source, function in the female, and ability to preserve sperm DNA integrity in the golden hamster. Journal of Andrology. 24(5):704-711. ISSN:1939-4640. http:/dx.doi.org/10.1002/j.1939-4640.2003.tb02730.x

DOMÍNGUEZ-REBOLLEDO AE, Fernando-Santos MR, Bisbal A, Ros-Santaella JL, Ramon M, Carmona M, Martínez-Pastor F, Garde JJ. 2010. Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments. Reproduction and Fertility and Development. 22(5): 856-870. ISSN: 1031-3613. http:/dx.doi.org/10.1071/RD09197

DOMÍNGUEZ-REBOLLEDO A, Sierra NL, Tamayo C A, Loria AA, Denis ES, Oses BR, Parra GE, Monsreal PL, Ugalde RJ. 2007. Fertility in hair sheep inseminated with freeze spermatozoa rediluted with seminal plasma. Revista Científica. 2007;17:73-76. http://produccioncientificaluz.org/index.php/cientifica/article/view/15259/15234

GILMORE J, MC Gann l, Ashwoth E, Acker J, Raath J, Bush M, Critser J. 1998. fundamental cryobiology of selected african mammalian spermatozoa and its role in biodiversity preservation through the development of genome resource banking. Animal Reproduction Science. 53(1-4): 277-297. ISSN: 0378-4320. http:/dx.doi.org/10.1016/S0378-4320(98)00118-3

HALLIWEL B. 1994. Free radicals and antioxidants: a personal view. Nutrition Reviews. 52(8): 253-265. ISSN: 0029-6643. http:/dx.doi.org/10.1111/j.1753-4887.1994.tb01453.x

HALLIWELL B. 1997. Antioxidants: The basics-what they are and how to evaluate them. Advances in Pharmacology. 38: 3-20. ISSN: 1054-3589. http:/dx.doi.org/10.1016/S1054-3589(08)60976-X

KANKOFER M, Kolm G, Aurich J, Aurich C. 2005. Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5 ºC. Theriogenology. 63(5): 1354-1365. ISSN: 0093-691X: http:/dx.doi.org/10.1016/j.theriogenology.2004.07.005

LERMEN D, Blömeke B, Browne R, Clarke A, Dyce PW, Fixemer T, Fuhr GR, Holt WV, Jewgenow K, Lloyd RE, Lotters S, PaulusM, Reid GM, Rapoport DH, Rawson D, Ringleb J, Ryder OA, Sporl G, Schmitt T, Veith M, Muller P. 2009. Cryobanking of viable biomaterials: Implementation of new strategies for conservation purposes. Molecular Ecology. 18(6): 1030-1033. ISSN: 1365-294X. http:/dx.doi.org/10.1111/j.1365-294X.2008.04062.x

MATA-CAMPUZANO M, Álvarez-Rodríguez M, Álvarez M, Tamayo-Canul J, Anel L, Paz P, Martínez-Pastor F. 2015. Post-Thawing Quality and Incubation Resilience of Cryopreserved Ram Spermatozoa are Affected by Antioxidant Supplementation and Choice of Extender. Theriogenology. 83(4): 520-528. ISSN: 0093-691X. http:/dx.doi.org/10.1016/j.theriogenology.2014.10.018

MEHDIPOUR M, Kia HD, Najafi A. 2019. Effect of Crocin and Naringenin Supplementation in Cryopreservation Medium on Post-Thawed Rooster Sperm Quality and Expression of Apoptosis Associated Genes. Biorxiv. 1-26. https://doi.org/10.1101/846758

MICHIELS C, Raes M, Toussaint O, Remacle J. 1994. Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress. Free Radicals Biology. 17(3): 235-248. ISSN: 0891-5849. http:/dx.doi.org/10.1016/0891-5849(94)90079-5

PEÑA FJ, Johannison A, Wallgren M, Rodríguez-Martinez H. 2005. A new and simple method to evaluate early membrane changes in frozen- thawed boar spermatozoa. International journal of Andrology. 28(2): 107-114.ISSN:1939-4640: http:/dx.doi.org/10.1111/j.1365-2605.2005.00512.x

SALAMON S, Maxwell WM. 2000. Storage of ram semen. Animal Reproduction Science. 62(1-3): 77-111. ISSN: 0378-4320. http:/dx.doi.org/10.1016/s0378-4320(00)00155-x

SAPANIDOU VG, Margaritis L, Siahos N, Arsenopoulos K, Dragatidou E, Taitzoglou LA, Zervos LA, Theodoridis A, Tsantarliotou MP. 2014. Antioxidant effect of a polyphenol-rich grape pomace extract on motility, viability and lipid peroxidation of thawed bovine spermatozoa. Journal of Biological Research. 21(19): 1-6. ISSN: 2284-0230. http:/dx.doi.org/10.1186/2241-5793-21-19

SCHMEHL MK, Vazquez I, Graham EF. 1986. the effects of nonpenetrating cryoprotectans added to test-yolk-glycerol extender on the post-thawing motility of ram spermatozoa. Cryobiology. 23(6): 512-517. ISSN: 0011-2240. http:/dx.doi.org/10.1016/0011-2240(86)90060-X

S.A.S. Institute Inc. 2003. SAS/STAT user´s Guide. Version 6. Fourth Edition. Vol. 1. Carry, NC.

THUWANUT P, Chatdarong K, Techakumphu, M, Axner E. 2008. The effect of antioxidants on motility, viability, acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa. Theriogenology. 70(2): 233-240. ISSN: 0093-691X. http:/dx.doi.org/10.1016/j.theriogenology.2008.04.005

TREMELLEN K. 2008. Oxidative stress and male infertility: a clinical perspective. Human Reproduction. 14(3): 243-258. ISSN: 0268-1161. http:/dx.doi.org/10.1093/humupd/dmn004

WATSON PF. 2000. The causes of reduced fertility with cryopreserved semen. Animal reproduction Science. 60-61: 481-492. ISSN: 0378-4320. http:/dx.doi.org/10.1016/S0378-4320(00)00099-3

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