Efecto de los antioxidantes Trolox y Crocina sobre la criopreservación del semen de ovino Pelibuey

Itzel Rodríguez-Gutiérrez, Álvaro Domínguez-Rebolledo, Henry Loeza-Concha, Carlos Castellanos-Zacarías, Maximiliano Tun-Moo, Julio Ramón-Ugalde

Resumen

El objetivo del estudio fue evaluar el efecto de los antioxidantes Trolox y Crocina en el diluyente de congelación sobre la criopreservación del semen de ovino Pelibuey. Los eyaculados obtenidos se mezclaron, dividieron y congelaron en 3 tratamientos: T1: Trolox 1mM; T2: Crocina 1mM; TT: Testigo (sin antioxidante). Se analizó a las 0 horas (descongelación) y 6 horas a 37 °C, la motilidad total (MT) y progresiva (MP), la viabilidad espermática, la actividad mitocondrial, la integridad de los acrosomas y de la membrana de la cola (Host). Los datos se analizaron con un ANOVA para un diseño completamente al azar y una prueba de Tukey para la comparación de medias. En ninguna de las dos horas se encontraron diferencias significativas en los tratamientos (P>0.05). Sin embargo, de 0 a 6h, la MT se comportó de forma similar en el T1 y T2, pero no así en el TT (P<0.05), por el contrario, la integridad de los acrosomas en los tres tratamientos fue similar (P<0.05). La MP, viabilidad, actividad mitocondrial y Host disminuyeron con el tiempo de forma similar en los tres tratamientos. Los antioxidantes Trolox y Crocina a 1mM en el diluyente de congelación, permiten mantener la MT y la integridad de los acrosomas durante 6 h de incubación a 37 °C.

Palabras clave

Radicales libres, diluyente, esperma y congelación

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