Determinación de ácidos grasos no esterificados, β-hidroxibutirato, triacilglicerol y colesterol durante el balance energético negativo en vacas Holstein

Omar Prado-Rebolledo, Gustavo Valpuesta-Santos, Favio Valencia-Magaña, Juan Hernández-Rivera, Rafael Macedo-Barragán, Arturo García-Casillas

Resumen

El objetivo del presente trabajo fue cuantificar la concentración de ácidos grasos no esterificados, β-hidroxibutirato, triacilglicerol y colesterol durante el balance energético negativo, en vacas Holstein. El estudio se llevó a cabo mediante el muestreo y análisis de 250 sueros sanguíneos obtenidos de 50 vacas Holstein. Después del parto, el primer muestreo se realizó a los 7 d, el segundo a los 15 d y los tres restantes a los 30, 45 y 60 d. Valores medios para ácidos grasos no esterificados, β-hidroxibutirato, triacilglicerol y colesterol fueron determinados. El conjunto de datos resultante se analizó mediante distribución de Gauss y estadística descriptiva. Se establecieron intervalos de confianza del 95%. La comparación entre grupos se llevó a cabo por Análisis de Varianza e histograma de frecuencia. Los ácidos grasos no esterificados, el β-hidroxibutirato y el triacilglicerol, son consistentes con los valores reportados internacionalmente. La concentración de colesterol mostró un valor ligeramente superior a las referencias. Los AGNE no presentaron diferencias entre grupos, el β-hidroxibutirato registró su valor más alto a los 15 d posparto, y más bajo a los 60 d posparto. El triacilglicerol y colesterol mostraron su concentración más alta a los 60 d posparto, y la más baja a los 15 d posparto. Los intervalos de confianza calculados se podrían utilizar a nivel hato para detectar situaciones de alerta cuando al menos 5 % de las vacas en la muestra se sitúen fuera del intervalo de referencia.

Palabras clave

Perfiles metabólicos; ácidos grasos no esterificados; β-hidroxibutirato; triacilglicerol; colesterol; vacas lecheras

Texto completo:

PDF PDF (English) XML-JATS

Referencias

ABDELLI A, Raboisson D, Kaidi R, Ibrahim B, Kalem A, Iguer-Ouada M. 2017. Elevated non-esterified fatty acid and β-hydroxybutyrate in transition dairy cows and their association with reproductive performance and disorders: a meta-analysis. Theriogenology. 93(1):99-104. ISSN: 1879-3231 (Electronic) 0093-691X (Linking). DOI: 10.1016/j.theriogenology.2017.01.030.

ARGÜESO AR, Díaz DJL, Díaz PJA, Rodríguez GA, Castro MM, Diz LF. 2011. Lipids, cholesterol and lipoproteins. Galicia Clin. 72(suppl):7-17. ISSN: 0304-4866. DOI: Not available.

CONTRERAS GA, Strieder BC, De Koster J. 2018. Modulating adipose tissue lipolysis and remodeling to improve immune function during the transition period and early lactation of dairy cows. J Dairy Sci. 101(3):2737-2752. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2017-13340.

DA FONSECA LLF, Rodríguez MPH, dos Santos VM, Lima PA, Lucci SC. 2004. Supplementation of dairy cows with propylene glycol during the periparturient period: effects on body condition score, milk yield, first estrus post-partum, β-hydroxybutyrate, non-esterified fatty acids and glucose concentrations. Cienc Rural. 34(3):897-903. ISSN: 1678-4596 (Electronic). DOI: 10.1590/S0103-84782004000300037.

DOREA JRR, French EA, Armentano LE. 2017. Use of milk fatty acids to estimate plasma nonesterified fatty acid concentrations as an indicator of animal energy balance. J Dairy Sci . 100(8):6164-6176. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2016-12466.

DUSKE K, Hammon HM, Langhof AK, Bellmann O, Losand B, Nurnberg K, Nurnberg G, Sauerwein H, Seyfert HM, Metges CC. 2009. Metabolism and lactation performance in dairy cows fed a diet containing rumen-protected fat during the last twelve weeks of gestation. J Dairy Sci . 92(4):1670-1684. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2008-1543.

FOLNOŽIĆ I, Turk R, Đuričić D, Vince S, Pleadin J, Flegar-Meštrić Z, Valpotić H, Dobranić T, Gračner D, Samardžija M. 2015. Influence of Body Condition on Serum Metabolic Indicators of Lipid Mobilization and Oxidative Stress in Dairy Cows During the Transition Period. Reprod Domest Anim. 50(6):910-917. ISSN: 1439-0531 (Electronic) 0936-6768 (Linking). DOI: 10.1111/rda.12608.

GARCÍA CAC, Montiel RLA, Borderas TF, Girard V. 2015. Relationship between β-hydroxybutyrate and fat:protein ratio of milk during early lactation in dairy cows. Arch Med Vet 47(1):21-25. ISSN: 0719-8132 (Electronic) 0719-8000 (Linking). DOI: 10.4067/S0301-732X2015000100005.

GUYOT H, Detilleux J, Lebreton P, Garnier C, Bonvoisin M, Rollin F, Sandersen C. 2017. Comparison of various indices of energy metabolism in recumbent and healthy dairy cows. PLoS One. 12(1):e0169716-e0169726. ISSN: 1932-6203 (Electronic) 1932-6203 (Linking). DOI: 10.1371/journal.pone.0169716.

IMHASLY S, Bieli C, Naegeli H, Nystrom L, Ruetten M, Gerspach C. 2015. Blood plasma lipidome profile of dairy cows during the transition period. BMC Vet Res. 11(1):252-259. ISSN: 1746-6148 (Electronic) 1746-6148 (Linking). DOI: 10.1186/s12917-015-0565-8.

JUMP DB. 2011. Fatty acid regulation of hepatic lipid metabolism. Curr Opin Clin Nutr Metab Care. 14(2):115-120. ISSN: 1473-6519 (Electronic) 1363-1950 (Linking). DOI: 10.1097/MCO.0b013e328342991c.

KANEKO JJ, Harvey WJ, Bruss LM. 2008. "Appendix VIII Blood Analyte Reference Values in Large Animals". In: Kaneko JJ, Harvey WJ , Bruss LM, Clinical Biochemistry of Domestic Animals. California, Estados Unidos: Academic Press. 882-888 p. ISBN: 978-0-12-370491-7.

LUCEY JA, Otter D, Horne DS. 2017. A 100 year review: progress on the chemistry of milk and its components. J Dairy Sci . 100(12):9916-9932. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2017-13250.

MANI O, Sorensen MT, Sejrsen K, Bruckmaier RM, Albrecht C. 2009. Differential expression and localization of lipid transporters in the bovine mammary gland during the pregnancy-lactation cycle. J Dairy Sci . 92(8):3744-3756. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2009-2063.

MANI O, Korner M, Ontsouka CE, Sorensen MT, Sejrsen K, Bruckmaier RM, Albrecht C. 2011. Identification of ABCA1 and ABCG1 in milk fat globules and mammary cells--implications for milk cholesterol secretion. J Dairy Sci . 94(3):1265-1276. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2010-3521.

NRC. 2001. National Research Council. Nutrient Requirements of Dairy Cattle. 7th ed. National Academy Press, Washington DC., USA.

OETZEL GR. 2004. Monitoring and testing dairy herds for metabolic disease. Vet Clin North Am Food Anim Pract 20(3):651-674. ISSN: 0749-0720 (Electronic). DOI: 10.1016/j.cvfa.2004.06.006.

PEEL MC, Finlayson BL, McMahon TA. 2007. Updated world map of the Köppen-Geiger climate classification. Hydrol Earth Syst Sci. 11(5):1633-1644. ISSN: 1027-5606 (Electronic) 1607-7938 (Linking). DOI: 10.5194/hess-11-1633-2007, 2007.

RUOFF J, Bertulat S, Burfeind O, Heuwieser W. 2016. Associations of β-hydroxybutyrate, cholesterol, triglycerides and high-density lipoproteins to non-esterified fatty acids pre- and postpartum. J Dairy Res. 83(4):447-452. ISSN: 1469-7629 (Electronic) 0022-0299 (Linking). DOI: 10.1017/S0022029916000534.

SAS Institute. 2010. Statistical Analysis Software SAS/STAT®. version 9.0.2, Cary, N.C., USA: SAS Institute Inc., ISBN: 978-1-60764-599-3. Available: http://www.sas.com/en_us/software/analytics/stat.html

SHEEHY MR, Fahey AG, Aungier SPM, Carter F, Crowe MA, Mulligan FJ. 2017. A comparison of serum metabolic and production profiles of dairy cows that maintained or lost body condition 15 days before calving. J Dairy Sci . 100(1):536-547. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2016-11206.

SOLBERG HE. 1987. Approved recommendation (1987) on the theory of reference values. Part 5. Statistical treatment of collected reference values. Determination of reference limits. Clin Chim Acta. 170(2-3):S13-S32. ISSN:1873-3492 (Electronic) 0009-8981 (Linking) DOI: 10.1016/0009-8981(87)90151-3.

SUN F, Cao Y, Cai C, Li S, Yu C, Yao J. 2016. Regulation of nutritional metabolism in transition dairy cows: energy homeostasis and health in response to post-ruminal choline and methionine. PLoS One . 11(8):e0160659-e0160669. ISSN: 1932-6203 (Electronic) 1932-6203 (Linking). DOI: 10.1371/journal.pone.0160659.

VAN HOEIJ RJ, Dijkstra J, Bruckmaier RM, Gross JJ, Lam T, Remmelink GJ, Kemp B, van Knegsel ATM. 2017. The effect of dry period length and postpartum level of concentrate on milk production, energy balance, and plasma metabolites of dairy cows across the dry period and in early lactation. J Dairy Sci . 100(7):5863-5879. ISSN: 1525-3198 (Electronic) 0022-0302 (Linking). DOI: 10.3168/jds.2016-11703.

WAKIL SJ, Abu-Elheiga LA. 2009. Fatty acid metabolism: target for metabolic syndrome. J Lipid Res 50(Suppl):138-143. ISSN: 1539-7262 (Electronic) 0022-2275 (Linking). DOI: 10.1194/jlr.R800079-JLR200.

ZÁRATE-MARTÍNEZ JP, Vinay-Vadillo JC, Carballo CO, Hernández-Hernández VD, Amezcua-Manjarres EV. 2011. Efecto de la alimentación con grasas protegidas en vacas de doble propósito. Agron Mesoam. 22(2):359-366. ISSN: 1021-7444.

Enlaces refback

  • No hay ningún enlace refback.