Addition of exogenous enzymes in diets for laying hens on performance
DOI:
https://doi.org/10.21929/abavet2026.9Keywords:
phytases, laying hens, productive performanceAbstract
The enzymes xylanase, amylase, protease, and phytase were evaluated in chicken diets for their effect on productive performance. 432 Bovans hens were used in six treatments arranged in a 2x3 factorial design, with six replicates of 12 chickens each. Factor A was diets with 17% or 15% crude protein (CP) and -150 kcal/kg of metabolizable energy (ME); the factor B was diets with and without 600 phytic units (FTU) of phytases and the addition of XAP (xylanases, amylases, and proteases). The results obtained in eight weeks of experimentation showed no difference (P>0.05) between the diets with and without phytases; the latter resulted in a reduction of 0.16% in calcium, 0.18% in phosphorus, 75 kcal/kg of ME, 0.81% in protein, and 0.037% in amino acids. Egg weight and feed conversion ratio were affected (P<0.05) in the birds that received the diets with 15% CP. The inclusion of XAP improved the percentage of egg production (P<0.05), egg weight, and feed conversion ratio (P<0.05) in the diets with less protein and ME. Shell strength increased (P<0.05) in diets with phytases. The use of XAP and phytases decreased the inclusion of soybean meal, orthophosphate, oil, and salt in the diet.http://dx.doi.org/10.21929/abavet2026.9
e2026-101
References
ABDOLLAHI A, Karimi A, Sadeghi AA, Bedford MR, Ashengroph M. 2021. The effects of the fiber source and xylanase supplementation on production, egg quality, digestibility, and intestinal morphology in the aged laying hen. Poultry Science. 100(3):100936. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2020.12.033
ARABSHAAHI HA, Ghasemi HA, Hajkhodadadi I, Farahani AHK. 2021. Effects of multicarbohydrases and butyrate glycerides on productive performance, nutrient digestibility, gut morphology and ileal microbiota in late- phase laying hens fed corn- or wheat-based diets. Poultry Science. 100(5):101066. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2021.101066
BELLO A, Giménez-Rico RD, Gilani S, Hillen BC, Venter KM, Plumstead P, Dersjant-Li Y. 2023. Application of enzyme matrix values for energy and nutrients to a wheat-corn-soybean meal-based broiler diet supplemented with a novel phytase, with or without a xylanase-B-glucanase, achieved a production benefit over a nutritionally adequate unsupplemented diet. Poultry Science. 102:1-9. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2023.103131
BOUGOUIN A, Appuhamy JADRN, Kebreab E, Dijkstra J, Kwakkel RP, France J. 2014. Effects of phytase supplementation on phosphorus retention in broilers and layers; A meta-analysis. Poultry Science. 93: 981-1992. ISSN: 0032-5791. https://doi.org/10.3382/ps.2013-03820
ELTAHAN HM, Cho S, Rana MM, Saleh AA, Elkomi AE, Wadaan MAM, Alagawany M, Kim IH, Eltahan HM. 2023. Dietary exogenous phytase improves egg quality, reproductive hormones, and prolongs the lifetime of the aging Hy-Line brown laying hens fed nonphytate phosphorus. Poultry Science. 102(9):102895. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2023.102895
Facultad de Medicina Veterinaria y Zootecnia (FMVZ). 2026. Localización geográfica del C.E.I.E.P.Av.-Tláhuac, CDMX. https://www.fmvz.unam.mx/fmvz/centros/ceiepav/localizacion.html
FRØLICH W. 1990. Chelating properties of fiber and phytate. New developments in dietary fiber: physiological, physicochemical and analytical aspects. I. Furda and C. J. Brine, ed. Premium Press, New York, NY. Pp. 83-93. ISBN: 0306435837. https://link.springer.com/book/10.1007/978-1-4684-5784-1
GORENZ B, Iseri V, Rubach J, Dilger RN. 2022. Xylanases supplementation of pelleted wheat-based diets increases growth efficiency and apparent metabolizable energy and decreases viscosity of intestinal contents in broilers. Poultry Science. 101:1-10. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2022.102220
HERVO F, Létourneau-Montminy MP, Meme N, Méda B, Duelos MJ, Narcy A. 2023. Effect of phytase and limestone particle seze on mineral digestibility, performance, eggshell quality, and bene mineralization in laying hens. Poultry Science. 102(5):102613. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2023.102613
LIU H, Faruk MU, Teixeira L, Fávero A, Vieira SL. 2024. Research Note: Proteases effect on soybean meal: enzyme product and area of production differences on standardized ileal amino acids digestibility of broiler chickens. Poultry Science. 103:1-9. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2024.104493
MORGAN NK(a), Bhuiyan MM, Hopcroft R. 2022. Non-starch polysaccharide degradation in the gastrointestinal tract of broiler chickens fed commercial-type diets supplementation with either a single dose of xylanase, a double dose of xylanase, or a cocktail of non-starch polysaccharide-degradins enzymes. Poultry Science. 101:1-8. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2022.101846
MORGAN NK(b), Wallace A, Bedford MR, González-Ortiz. 2022. Impact of fermentable fiber, xylo-oligosaccharides and xylanase on layin hen productive performance and nutrient utilization. Poultry Science. 101:1-9. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2022.102210
MOURA TF, Reis MP, Horna FA, Nóbrega IPT, Bello A, Donato DCZ, White E, Li YD, Sakomura NK. 2023. A novel consensus bacterial 6-phytase variant improve the responses of laying hens fed an inorganic phosphorus-free diet with reduced energy and nutrients from 23-72 wk of age. Poultry Science. 102(10):102949. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2023.102949
PARK J, Yun-Ji H, Da-Hye K, Kim YB, Byung-Yeon K, Ju-Young S, Kyung-Woo L. 2024. Nutritional and physiological responses to dietary phosphorus levels and phytase in pullets and laying hens. Poultry Science. 103:1-9. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2024.103886
PARKKOEN TA, Tervila-Wiko M, Hopeakoski-Nurminen A, Morgan K, Poutanen KA. 1997. Changes in wheat microstructure following in vitro digestion. Acta Agriculturae Scandinavica, Section B — Soil & Plant Science. 47:43–47. ISSN: 0906-4710. https://doi.org/10.1080/09064719709362437
PONGMANCE K, Kuhn I, Korver DR. 2020. Effects of phytase supplementation on eggshell and bone quality, and phosphorus and calcium digestibility in laying hens from 25 to 37 wk of age. Poultry Science. 99(5):2595-2607. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2019.12.051
POUDEL I, Hodge VR, Wamsley KGS, Roberson KD, Adhikari PA. 2023. Effects of protease enzyme supplementation and varyng levels of amino acid inclusion of productive performance, egg quality, and amino acid digestibility in laying hens from 30 to 50 weeks of age. Poultry Science. 102(3):102465. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2022.102465
RAVINDRAN V. 2013. Feed enzymes: the science, practice, and metabolic realities 1. The Journal of Applied Poultry Research. 22(3): 628–636. ISSN: 1056-6171. https://doi.org/10.3382/japr.2013-00739
SCHRAMM VG, Massuquetto A, Bassi LS, Zavelinski VAB, Sorbara JOB, Cowieson AJ, Felix AP, Maiorka A. 2021. Exogenous α-amylase improves tha digestibility of corn and corn-soybean meal diets for broilers. Poultry Science. 100:1-1-8. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2021.101019
SOMMERFELD V, Huber K, Bennewitz J, Camarinha-Silva A, Hasselmann M, Ponsuksili S, Seifert J, Wimmers K, Rodehutscord M. 2020. Phytase degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods. Poultry Science. 99(12): 6797-6808. ISSN: 0032-5791. https://doi.org/10.1016/j.psj.2020.08.064
STEFANELLO C, Vieira SL, Soster P, Dos Santos BM, Dalmoro YK, Facero A, Cowieson AJ. 2019. Utilization of corn-based diets supplemented with an exogenous α-amylase for broilers. Poultry Science. 98:5862-5869. ISSN: 0032-5791. https://doi.org/10.3382/ps/pez290
